A computational analysis of FXa generation by TF:FVIIa on the surface of rat vascular smooth muscle cells

• Published in: Annals of biomedical engineering Vol. 26; no. 1; pp. 28 - 36
• Main Authors: HALL, C. L, SLACK, S. M, TURITTO, V. T
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.01.1998; Springer Nature B.V
• Subjects: Algorithms; Animals; Biological and medical sciences; Biological Transport - physiology; Blood coagulation. Blood cells; Cells, Cultured; Coagulation factors; Computer simulation; Computer software; Convection; Diffusion; Diffusion in solids; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Endothelium, Vascular - physiology; Experimental data; Factor VIIa - physiology; Factor Xa - biosynthesis; Finite Element Analysis; Fundamental and applied biological sciences. Psychology; Hemorheology; Mass transfer; Mass transport; Models, Cardiovascular; Models, Chemical; Molecular and cellular biology; Muscle; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - physiology; Numerical Analysis, Computer-Assisted; Physiological models; Proteins; Rats; Reaction kinetics; Reproducibility of Results; Shear flow; Space life sciences; Thromboplastin - physiology; Vertebrata; Factor X; Mammalia; Shear; Rat; Animal; Rodentia; Coagulation; Tissue factor; Smooth muscle; Numerical simulation; Activation
• ISSN: 0090-6964; 1573-9686
• DOI: 10.1114/1.120

A computational model was developed to investigate the contribution of classical mass transport and flow parameters to factor X (FX) activation by the tissue factor-factor VIIa complex (TF:VIIa) on one wall of a parallel-plate flow chamber. The computational results were compared to previously obtained experimental data for the generation of factor Xa (FXa) by TF:VIIa on the surface of cultured rat vascular smooth muscle cells. In this study, the complete steady-state convection-diffusion equation was solved using the commercial software package, FLUENT (Fluent Inc., Lebanon, New Hampshire). A user-defined subroutine interfaced with FLUENT implemented the surface reaction which was modeled using classical Michaelis-Menten reaction kinetics. The numerical solutions were obtained for 12 cases which used combinations of three wall shear rates and four reaction rates. The numerically obtained fluxes for a given reaction rate displayed a wall shear rate dependence which ranged from classical kinetic reaction control (no dependence) to pure diffusional control (maximum dependence). The experimental data, however, were not represented by numerical data generated using a single reaction rate. The three numerically obtained fluxes which corresponded most closely to the experimental fluxes were determined using three different Vmax values. This finding supports the hypothesis that there may be a direct effect of flow on the TF:VIIa complex or the cell membrane.