Microwave seed priming and ascorbic acid assisted phytoextraction of heavy metals from surgical industry effluents through spinach

• Published in: Ecotoxicology and environmental safety Vol. 282; p. 116731
• Main Authors: Abubakar, Muhammad, Alghanem, Suliman Mohammed Suliman, Alhaithloul, Haifa Abdulaziz Sakit, Alsudays, Ibtisam Mohammed, Farid, Mujahid, Zubair, Muhammad, Farid, Sheharyaar, Rizwan, Muhammad, Yong, Jean Wan Hong, Abeed, Amany H.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.09.2024; Elsevier
• Subjects: Accumulation; Antioxidant enzymes; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; Bioremediation; Biosanering; Botanik; Botany; Heavy metals; Photosynthetic pigments; Phyto-treatment; phytoremediation; Reactive oxygen species; Reactive oxygen species; Phyto-treatment; Antioxidant enzymes; phytoremediation; Photosynthetic pigments; Heavy metals; Accumulation
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116731

The prevalence of inorganic pollutants in the environment, including heavy metals (HMs), necessitates a sustainable and cost-effective solution to mitigate their impacts on the environment and living organisms. The present research aimed to assess the phytoextraction capability of spinach (Spinach oleracea L.), under the combined effects of ascorbic acid (AA) and microwave (MW) irradiation amendments, cultivated using surgical processing wastewater. In a preliminary study, spinach seeds were exposed to MW radiations at 2.45 GHz for different durations (15, 30, 45, 60, and 90 seconds). Maximum germination was observed after the 30 seconds of radiation exposure. Healthy spinach seeds treated with MW radiations for 30 s were cultivated in the sand for two weeks, after which juvenile plants were transferred to a hydroponic system. Surgical industry wastewater in different concentrations (25 %, 50 %, 75 %, 100 %) and AA (10 mM) were provided to both MW-treated and untreated plants. The results revealed that MW-treatment significantly enhanced the plant growth, biomass, antioxidant enzyme activities and photosynthetic pigments, while untreated plants exhibited increased reactive oxygen species (ROS) and electrolyte leakage (EL) compared with their controls. The addition of AA to both MW-treated and untreated plants improved their antioxidative defense capacity under HMs-induced stress. MW-treated spinach plants, under AA application, demonstrated relatively higher concentrations and accumulation of HMs including lead (Pb), cadmium (Cd) and nickel (Ni). Specifically, MW-treated plants with AA amendment showed a significant increase in Pb concentration by 188 % in leaves, Cd by 98 %, and Ni by 102 % in roots. Additionally, the accumulation of Ni increased by 174 % in leaves, Cd by 168 % in roots, and Pb by 185 % in the stem of spinach plant tissues compared to MW-untreated plants. These findings suggested that combining AA with MW irradiation of seeds could be a beneficial strategy for increasing the phytoextraction of HMs from wastewater and improving overall plant health undergoing HMs stress. [Display omitted] •Low level microwave (MW) radiations increased seed germination and growth of spinach.•Surgical industrial wastewater differentially affected the growth of MW treated and untreated spinach.•Ni, Cd and Pb concentrations increased in spinach with increasing concentrations of surgical wastewaters.•Ascorbic acid improved the physiology and biochemistry of MW treated and untreated spinach.•Ascorbic acid increased Ni, Cd and Pb accumulation in both MW treated and untreated spinach grown under wastewater.