Optimization Production of Biosurfactant by Pseudomonas putida Using Crude Palm Oil (CPO) as Substrate

The production of biosurfactant by Pseudomonas putida has been studied. P. putida FNCC 0071 was grown in the nutrient broth medium supplemented with NaCl and crude palm oil (CPO). The effect of CPO concentration and fermentation time on the biosurfactant production were evaluated. The biosurfactant...

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Bibliographic Details
Published inIOP conference series. Earth and environmental science Vol. 75; no. 1; pp. 12013 - 12017
Main Authors Suryanti, V, Handayani, D S, Masykur, A, Lindasari
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.07.2017
Subjects
biosurfactants
Emulsification
Fermentation
Optical density
Optimization
Palm oil
Pseudomonas putida
Rhamnolipids
Sodium chloride
Substrates
Surface tension
Surfactants
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Summary:The production of biosurfactant by Pseudomonas putida has been studied. P. putida FNCC 0071 was grown in the nutrient broth medium supplemented with NaCl and crude palm oil (CPO). The effect of CPO concentration and fermentation time on the biosurfactant production were evaluated. The biosurfactant production was evaluated every 24 h for 10 days by optical density, surface tension and emulsification index. The best culture medium was found to be medium containing 5% v/v of CPO with 5 days of incubation time. The biosurfactant was identified as rhamnolipids.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/75/1/012013