Expression and regulation of ROR-1 during early avian limb development

• Published in: Anatomy and Embryology Vol. 207; no. 6; pp. 495 - 502
• Main Authors: Rodriguez-Niedenführ, Marc, Pröls, Felicitas, Christ, Bodo
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01.03.2004
• Subjects: Amino Acid Sequence; Animals; Body Patterning - drug effects; Body Patterning - physiology; Bone Morphogenetic Proteins - pharmacology; Chick Embryo - physiology; Cloning, Molecular; Fibroblast Growth Factors - pharmacology; Hindlimb - drug effects; Hindlimb - embryology; Hindlimb - metabolism; Humans; In Situ Hybridization; Molecular Sequence Data; Nuclear Receptor Subfamily 1, Group F, Member 1; Receptor Protein-Tyrosine Kinases - genetics; Receptor Protein-Tyrosine Kinases - metabolism; Receptor Tyrosine Kinase-like Orphan Receptors; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Cytoplasmic and Nuclear; Sequence Homology, Amino Acid; Species Specificity; Trans-Activators; Tretinoin - pharmacology; Up-Regulation; Wings, Animal - drug effects; Wings, Animal - embryology; Wings, Animal - metabolism
• ISSN: 0340-2061; 1863-2653; 1432-0568; 0340-2061
• DOI: 10.1007/s00429-004-0381-6

ROR-1 is a member of the ROR family of tyrosine kinase like orphan receptors and is highly conserved among various species. We have isolated the chick ROR-1 ( cROR-1) and show that cROR-1 expression is high and restricted to the proximal limb region until HH-stage 25. At later stages, expression spreads towards the distal limb region. In order to determine the signals that control cROR-1 expression, factors known to be involved in limb patterning (FGFs, BMPs, SHH, retinoic acid) were applied to the developing limb. Whereas neither FGFs, BMPs, nor SHH affected cROR-1 expression, upregulation could be achieved by ectopic application of retinoic acid to the distal limb region. As retinoic acid also upregulated retinoic acid receptor beta ( Rar-beta), we assume that cROR-1 upregulation is mediated by Rar-beta. We conclude that ROR-1 signaling is an independently regulated pathway, which is involved in late rather than early limb development.