Protection of hepatic cell damage and antimicrobial evaluation of chitosan-catechin conjugate

• Published in: Applied biological chemistry Vol. 56; no. 6; pp. 701 - 707
• Main Authors: Cho, Young-Sook, Lee, Dae-Sung, Kim, Young-Mog, Ahn, Chang-Bum, Kim, Do-Hyung, Jung, Won-Kyo, Je, Jae-Young
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2013; Springer Nature B.V; 한국응용생명화학회
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Catechin; Cell viability; Chemistry; Chemistry and Materials Science; Chitosan; Conjugates; Damage assessment; Drug resistance; Foodborne pathogens; Free radicals; Glutathione; Hepatocytes; Hydrogen peroxide; Lipid peroxidation; Lipids; Liver; Methicillin; Original Article; Oxidative stress; Pathogens; Peroxidation; Reactive oxygen species; Staphylococcus aureus; Staphylococcus infections; 농학; Antimicrobial; Oxidative stress; Catechin; Chitosan; Antioxidant; Hepatotoxicity
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-013-3168-8

The chitosan-catechin conjugate was developed by free radical-induced conjugating reaction, and its protection ability against hydrogen peroxide-induced hepatic damage in human normal Chang liver cells and antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and foodborne pathogens were investigated. Treatment of hydrogen peroxide (650 μM) on Chang liver cells decreased cell viability up to 59.38% compared to the non-treatment group; however, cotreatment of the chitosan-catechin conjugate increased cell viability up to 76.90% at 200 μg/mL, and the protection ability was significantly higher than the unmodified chitosan ( p <0.05). The chitosan-catechin conjugate significantly ( p <0.05) inhibited the formation of intracellular reactive oxygen species and lipid peroxidation in Chang liver cells. Moreover, the chitosan-catechin conjugate increased glutathione levels in normal condition as well as under oxidative stress by hydrogen peroxide. Additionally, the chitosan-catechin conjugate showed increased antimicrobial activity against MRSA and foodborne pathogens as compared to those of the unmodified chitosan.