Characterization of a sialate pyruvate-lyase in the cytosol of human erythrocytes

• Published in: Biochimie Vol. 84; no. 7; pp. 655 - 660
• Main Authors: Bulai, Tatiana, Bratosin, Daniela, Artenie, Vlad, Montreuil, Jean
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.07.2002
• Subjects: Autoradiography; Carbon Radioisotopes; Catalysis; Cations - pharmacology; Chromatography, Thin Layer; Cytosol - enzymology; Erythrocytes; Erythrocytes - enzymology; Humans; Hydrogen-Ion Concentration; Kinetics; Mammalian lyases; Metals - pharmacology; N-Acetylneuraminic Acid - chemistry; N-Acetylneuraminic Acid - metabolism; Oxo-Acid-Lyases - blood; Pyruvic Acid - pharmacology; Red blood cells; Sialate pyruvate-lyase; Substrate Specificity; Temperature; Time Factors; PBS; RBC; Red blood cells; Sialate pyruvate-lyase; Neu5Ac; Erythrocytes; CMP; Mammalian lyases; ManNAc; Hb; TLC
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/S0300-9084(02)01436-0

Sialate pyruvate-lyases, also known as sialate aldolases (EC 4.1.3.3), reversibly catalyse the cleavage of free N-acetylneuraminic acids to form pyruvate and N-acetylmannosamine. These enzymes are widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol. They play an important role in the regulation of sialic acid metabolism by controlling the intracellular concentration of sialic acids of biosynthetic or exogenous origin, thus preventing the accumulation of toxic levels of this sugar. Application of an original colorimetric micromethod for N-acetylmannosamine determination, as well as the use of [4,5,6,7,8,9- 14C] N-acetylneuraminic acid, led us to evidence a cytosolic neuraminate aldolase activity in human red blood cells (RBCs) and then to define the main characteristics of this enzyme: Michaelis–Menten type, K m: 1.4 ± 0.05 mM, optimal pH: 7.6 ± 0.2, optimal temperature: 70 ± 2 °C, inhibition by heavy metals: Ag + and Hg ++. These enzyme parameters are close to those of the bacterial and mammalian aldolases described up to now. At the moment, the presence of sialate pyruvate-lyase in the cytosol of red blood cells remains an enigma.