Evaluation of antibacterial efficacy of phyto fabricated silver nanoparticles using Mukia scabrella (Musumusukkai) against drug resistance nosocomial gram negative bacterial pathogens

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 104; pp. 282 - 288
• Main Authors: Prabakar, Kandasamy, Sivalingam, Periyasamy, Mohamed Rabeek, Siyed Ibrahim, Muthuselvam, Manickam, Devarajan, Naresh, Arjunan, Annavi, Karthick, Rajamanickam, Suresh, Micky Maray, Wembonyama, John Pote
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2013
• Subjects: Acinetobacter; Acinetobacter - drug effects; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; antibacterial properties; bacterial infections; colloids; color; Cucumis maderaspatanus; Dose-Response Relationship, Drug; Drug resistance; Drug Resistance, Bacterial - drug effects; energy; energy-dispersive X-ray analysis; Fourier transform infrared spectroscopy; Gram-negative bacteria; Klebsiella pneumoniae; Klebsiella pneumoniae - drug effects; leaf extracts; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Mukia scabrella; multiple drug resistance; nanomedicine; nanoparticles; nanosilver; Nosocomial infections; pathogens; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plants, Medicinal - chemistry; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; respiratory tract diseases; silver; Silver - chemistry; Silver - metabolism; Silver - pharmacology; Silver nanoparticles; Structure-Activity Relationship; surface plasmon resonance; transmission electron microscopy; ultraviolet-visible spectroscopy; X-radiation; X-ray diffraction; zeta potential; Antibacterial activity; Mukia scabrella; Nosocomial infections; Drug resistance; Silver nanoparticles
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2012.11.041

[Display omitted] ► A novel ayurvedic medicinal plant Mukia scabrella was exploited for the biosynthesis of AgNPs. ► AgNPs characterized using UV, FTIR, EDAX, XRD, TEM analysis and Zeta potential was measured. ► Antimicrobial activity was evaluated against drug resistance nosocomial gram negative bacterial pathogens. Given the fact in the limitation of the therapeutic options for emerging multidrug resistance gram-negative bacteria (MDR-GNB) of respiratory tract infections, the present study was focused on green synthesis of antimicrobial silver nanoparticles (AgNPs) using leaf extract of Mukia scabrella. An obvious color change to brown color and surface plasmon resonance by UV–visible spectroscopy (UV–vis) indicated a well observable peak at 440nm confirming the synthesis of AgNPs. Fourier transform infra-red spectroscopy (FTIR) analysis indicates protein as possible capping agents. Energy dispersive X-ray (EDAX) spectroscopy results showed major signal for elemental silver. X-ray diffraction (XRD) analysis indicates the formation of metallic silver nanomaterials. Transmission electron microscopic (TEM) study showed the nanoparticles in the size range of 18–21nm with spherical shape. Zeta potential analysis showed −21.7mV characteristic for stable AgNPs. The biosynthesized AgNPs exhibited significant antimicrobial activity against MDR-GNB nosocomial pathogens of Acinetobacter sp., Klebsiella pneumoniae and Pseudomonas aeruginosa. Results from the current study suggested that M. scabrella material could be exploited for the fabrication of AgNPs with potential therapeutic applications in nanomedicine especially for nosocomial bacterial infections.