Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules

• Published in: International journal of systematic and evolutionary microbiology Vol. 63; no. Pt 11; pp. 3950 - 3957
• Main Authors: De Meyer, Sofie E, Cnockaert, Margo, Ardley, Julie K, Maker, Garth, Yates, Ron, Howieson, John G, Vandamme, Peter
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.11.2013
• Subjects: bacteria; Bacterial Typing Techniques; Base Composition; Burkholderia; Burkholderia - classification; Burkholderia - genetics; Burkholderia - isolation & purification; chemotaxonomy; DNA, Bacterial - genetics; essential genes; Fabaceae - microbiology; fatty acid composition; Fatty Acids - chemistry; Genotype; Lebeckia; Likelihood Functions; Molecular Sequence Data; nucleic acid hybridization; Nucleic Acid Hybridization - genetics; Phylogeny; quinones; ribosomal RNA; RNA, Ribosomal, 16S - genetics; root nodules; Root Nodules, Plant - microbiology; Sequence Analysis, DNA; sequence homology; South Africa; Ubiquinone - chemistry
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.048777-0

Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005T being most closely related to Burkholderia tuberum (98.08 % sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA–DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005T = LMG 27175T = HAMBI 3357T) is proposed.