Humoral immune responses to adenovirus vectors in the brain

• Published in: Journal of neuroimmunology Vol. 103; no. 1; pp. 8 - 15
• Main Authors: Kajiwara, Koji, Byrnes, Andrew P, Ohmoto, Yoshinori, Charlton, Harry M, Wood, Matthew J.A, Wood, Kathryn J
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2000
• Subjects: Adenoviridae - genetics; Adenoviridae - immunology; Adenovirus; Adenovirus vectors; Animals; Antibodies, Viral - biosynthesis; Antibody; beta-Galactosidase - immunology; Brain; Brain - immunology; Gene transfer; Genetic Therapy; Genetic Vectors; Immune response; Immunohistochemistry; lacZ gene; Male; Mice; Mice, Inbred C3H; β-Galactosidase; Brain; Adenovirus vectors; Immune response; Gene transfer; Antibody; β-Galactosidase
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/S0165-5728(99)00220-9

We have investigated the humoral immune response to E1-deleted adenovirus vectors encoding the lacZ gene introduced into the brains of mice. Injection of these non-replicating vectors into the brain induced systemic antibody production to adenovirus vectors in dose dependent manner. Apparent antibody production to β-galactosidase, the product of the lacZ gene, was detected later than anti-adenovirus antibody. Neutralizing antibody was not detected. This study demonstrates that E1-deleted adenovirus vectors injected into the brain trigger humoral immune responses to the adenovirus and its gene products, but they are not sufficient to block the infection of cells by adenovirus upon repeat injection.