Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 531; pp. 45 - 47
Main Authors Abali, F., Stevens, M., Tibbe, A.G.J., Terstappen, L.W.M.M., van der Velde, P.N., Schasfoort, R.B.M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.08.2017
Subjects
Animals
Antibody/protein excretion
Cell Separation - instrumentation
Cell Separation - methods
EpCAM
Humans
Hybridoma
McSPRInter
Microwell
Single cells
SPR cytometry
SPRi
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Tissue Array Analysis - instrumentation
Tissue Array Analysis - methods
SPRi
EpCAM
Single cells
Microwell
Hybridoma
SPR cytometry
Antibody/protein excretion
McSPRInter
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Summary:Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2017.05.021