High-performance liquid chromatographic assay for the determination of 5-aminosalicylic acid and acetyl-5-aminosalicylic acid concentrations in endoscopic intestinal biopsy in humans

A high-performance liquid chromatographic method for the simultaneous determination of 5-amino-salicylic acid (5-ASA) and N-acetyl-5-ASA (Ac-5-ASA) concentrations in endoscopic mucosal biopsy homogenates is presented. The mean recoveries of 5-ASA and Ac-5-ASA from spiked blank biopsy homogenates ran...

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Bibliographic Details
Published inJournal of chromatography Vol. 564; no. 1; pp. 296 - 302
Main Authors De Vos, M., Verdievel, H., Schoonjans, R., Beke, R., De Weerdt, G.A., Barbier, F.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 08.03.1991
Subjects
Aminosalicylic Acids - analysis
Biopsy
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - statistics & numerical data
Endoscopy, Gastrointestinal
Humans
Intestinal Mucosa - chemistry
Mesalamine
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Summary:A high-performance liquid chromatographic method for the simultaneous determination of 5-amino-salicylic acid (5-ASA) and N-acetyl-5-ASA (Ac-5-ASA) concentrations in endoscopic mucosal biopsy homogenates is presented. The mean recoveries of 5-ASA and Ac-5-ASA from spiked blank biopsy homogenates ranged from 95.9 to 120% and from 92.5 to 100%, respectively. The coefficients of variation for 5-ASA and Ac-5-ASA were 0.7–8.6% and 1.4–12.9%, respectively. This method is useful for direct determination of topical availability of 5-ASA and Ac-5-ASA and probably an accurate parameter of drug bioavailability.
ISSN:0378-4347
DOI:10.1016/0378-4347(91)80094-S