Proteomics study of extracellular fibrinolytic proteases from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from Indonesian fermented food

This paper presents the proteomics study which includes separation, identification and characterization of proteins. The experiment on Indonesian fermented food such as extracellular fibrinolytic protease from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from red oncom and tempeh gem...

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Bibliographic Details
Published inIOP conference series. Earth and environmental science Vol. 55; no. 1; pp. 12025 - 12033
Main Authors Afifah, Diana Nur, Rustanti, Ninik, Anjani, Gemala, Syah, Dahrul, Yanti, Suhartono, Maggy T.
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.02.2017
Subjects
Bacillus licheniformis
Bacillus pumilus
Electrophoresis
Fermented food
Fibrin
Food
Mass spectrometry
Mass spectroscopy
Proteins
Proteomics
Tempeh
Two dimensional analysis
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Summary:This paper presents the proteomics study which includes separation, identification and characterization of proteins. The experiment on Indonesian fermented food such as extracellular fibrinolytic protease from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from red oncom and tempeh gembus was conducted. The experimental works comprise the following steps: (1) a combination of one- and two-dimensional electrophoresis analysis, (2) mass spectrometry analysis using MALDI-TOF-MS and (3) investigation using protein database. The result suggested that there were new two protein fractions of B. licheniformis RO3 and three protein fractions of B. pumilus 2.g. These result has not been previously reported.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/55/1/012025