A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-γ

Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-γ by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically...

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Bibliographic Details
Published inJournal of immunological methods Vol. 274; no. 1; pp. 229 - 232
Main Authors Puehler, Florian, Göbel, Thomas, Breyer, Ulrike, Ohnemus, Annette, Staeheli, Peter, Kaspers, Bernd
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.2003
Subjects
Animals
Bioassay
Biological Assay
Cell Line, Transformed
Chicken
Chickens - immunology
Dose-Response Relationship, Drug
Interferon-gamma - analysis
Interferon-gamma - genetics
Interferon-gamma - metabolism
Interferon-γ
Interleukin-18
Interleukin-18 - pharmacology
Kinetics
Sensitivity and Specificity
Transcription, Genetic
Chicken
Bioassay
Interleukin-18
Interferon-γ
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Summary:Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-γ by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-γ is quickly secreted in response to interleukin-18 exposure.
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SourceType-Scholarly Journals-1
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ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(02)00515-X