Lead availability and soil microbial community composition in rice rhizosphere affected by thiosulfate addition

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment Vol. 45; no. 3; pp. 232 - 237
• Main Authors: Lin, Huirong, Chen, Xincai, Hu, Shaoping, Shen, Chaofeng, Chen, Guangcun, Shi, Jiyan, Chen, Yingxu
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.07.2010; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: Agronomy. Soil science and plant productions; application rate; Availability; Biochemistry and biology; Biological and medical sciences; Chemical, physicochemical, biochemical and biological properties; clones; community structure; crop yield; flooded conditions; Fundamental and applied biological sciences. Psychology; lead; microbial activity; Microbiology; nutrient uptake; Oryza sativa; Paddy soil; paddy soils; PCR-DGGE; Physics, chemistry, biochemistry and biology of agricultural and forest soils; phytoremediation; phytotoxicity; plant growth; pollution control; rhizosphere; rice; Rice rhizosphere; roots; shoots; soil bacteria; soil pollution; soil respiration; Soil science; Sulfur; Thiobacillus; thiosulfates; Availability; PCR-DGGE; Pb; Rice rhizosphere; Sulfur; Paddy soil; Community structure; Microbial activity; Monocotyledones; Thiosulfates; Cultivated soil; Denaturing gradient gel electrophoresis; Ecology; Polymerase chain reaction; Rhizosphere; Oryza; Gramineae; Angiospermae; Herbaceous plant; Spermatophyta; Soil science; Microbial community
• ISSN: 0929-1393; 1873-0272
• DOI: 10.1016/j.apsoil.2010.04.010

A pot experiment was carried out to study the sulfur (S) and lead (Pb) interaction and its impact on soil microbial community composition in rice rhizosphere soil under flooded conditions. Paddy soil was treated with a Pb gradient with and without thiosulfate addition and then planted with rice. The increasing addition of Pb resulted in plant yield reduction and high, phytotoxic concentrations of Pb in roots with relatively low concentration of Pb in shoots. Under the impact of thiosulfate, Pb uptake in plants and NH 4OAc extractable Pb did not increase dramatically. PCR-DGGE experiment suggested that S action led to new bands. Specific clones (T3 and T6) found in S addition soils had high similarity to Thiobacillus, which indicated relatively high rates of potential S oxidation. S addition did not affect the availability Pb and the composition of soil microbial community. S addition is not a suitable amendatory tool of phytoremediation for Pb polluted soil.