Simple determination of peroxyl radical-trapping capacity

A simple spectrophotometric method of determination of peroxyl radical-trapping capacity (PRTC) of body fluids and food products is proposed. In this method, decomposition of 2,2'-azobis(2-amidopropane) hydrochloride (ABAP) is the source of peroxyl and alkoxyl radicals which oxidize 2,2'-a...

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Bibliographic Details
Published inBiochemistry and molecular biology international Vol. 46; no. 3; p. 519
Main Authors Bartosz, G, Janaszewska, A, Ertel, D, Bartosz, M
Format Journal Article
LanguageEnglish
Published England 01.10.1998
Subjects
Alcoholic Beverages
Amidines - metabolism
Amino Acids - pharmacology
Antioxidants - analysis
Antioxidants - pharmacology
Ascorbic Acid - analysis
Ascorbic Acid - pharmacology
Benzothiazoles
Chromans - analysis
Chromans - pharmacology
Epinephrine - pharmacology
Free Radicals
Indicators and Reagents
Oxidation-Reduction
Peroxides - metabolism
Plasma
Spectrophotometry
Sulfonic Acids - metabolism
Time Factors
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Summary:A simple spectrophotometric method of determination of peroxyl radical-trapping capacity (PRTC) of body fluids and food products is proposed. In this method, decomposition of 2,2'-azobis(2-amidopropane) hydrochloride (ABAP) is the source of peroxyl and alkoxyl radicals which oxidize 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to a green cation radical. Antioxidant present in a sample inhibit the reaction; the induction time of the reaction is proposed as a parameter enabling determination of antioxidant content. Standard assay conditions are: 20 mM ABAP and 150 microM ABTS in 0.1 M phosphate buffer, pH 7.0, at 37 degrees C; absorbance is monitored at 414 nm. A 10-min assay allows for determination of the induction time of appropriately diluted sample. As examples of application of this method, PRTC values of several types of beverages are reported.
ISSN:1039-9712
DOI:10.1080/15216549800204042