New Electroactive Hybridization Indicators 2-Phthalimido-N-Substitutedphenylethanesulfonamide Derivatives for Biosensor Applications: Ring Substituent Effect on Interaction between Compound and DNA

• Published in: Electroanalysis (New York, N.Y.) Vol. 22; no. 19; pp. 2225 - 2234
• Main Authors: Ozkan-Ariksoysal, Dilsat, Akgul, Ozlem, Aydinlik, Seyma, Topkaya, Seda Nur, Aladag, Nilay, Ozsoz, Mehmet
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.10.2010; WILEY‐VCH Verlag
• Subjects: 2-Phthalimido-N-substitutedphenylethanesulfonamides; Biosensors; Carbon; Carbon graphite electrode; Deoxyribonucleic acid; Derivatives; DNA; Drug-DNA interactions; Electrodes; Graphite; Guanine; Guanines; Hybridization; Indicators; Voltammetry
• ISSN: 1040-0397; 1521-4109; 1521-4109
• DOI: 10.1002/elan.201000128

In this work, an electrochemical DNA‐based sensor was developed for the detection of the interaction between the anticonvulsant compounds 2‐phthalimido‐N‐substituted phenylethanesulfonamides (PMPES‐derivatives) and 24‐mer short DNA sequences by using differential pulse voltammetry (DPV) based on both compound and guanine oxidation signals at the renewable carbon graphite electrode (CGE) surface. The influence of compounds on DNA showed differences depending on the nature and position of the substituent on the N‐phenyl ring. Compounds bearing 3‐methoxy, 4‐chloro and 2,6‐dimethyl substituents bind to single stranded probe DNA more strongly than the other derivatives of PMPES. Thus, these compounds were evaluated for use as an electrochemical hybridization label (indicator).