Conjugated linoleic acid decreases prostaglandin synthesis in bovine luteal cells in vitro

• Published in: Molecular reproduction and development Vol. 78; no. 5; pp. 328 - 336
• Main Authors: May, Katherine C.P., Bobe, Gerd, Mueller, Chad J., Cannon, Matthew J.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2011; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Cattle; Cells, Cultured; conjugated linoleic acid; corpus luteum; Cyclooxygenase 2 - biosynthesis; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 Inhibitors; Dairying; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Linoleic Acids, Conjugated - metabolism; Linoleic Acids, Conjugated - pharmacology; Luteal Cells - cytology; Luteal Cells - drug effects; Luteal Cells - metabolism; Mammalian female genital system; PGE2; PGF2α; Polymerase Chain Reaction; progesterone; Progesterone - biosynthesis; Prostaglandins - biosynthesis; RNA, Messenger - analysis; Vertebrates: reproduction; Conjugated linoleic acid; Bovine; Prostaglandin; Arachidonic acid derivatives; Ox; Biosynthesis; In vitro; Ovary; Vertebrata; Mammalia; Decrease; Luteal cell; Eicosanoid; Animal; Female genital system; Corpus luteum; Artiodactyla; Ungulata
• ISSN: 1040-452X; 1098-2795
• DOI: 10.1002/mrd.21308

Feeding conjugated linoleic acids (CLA) improves reproductive performance in dairy cows; however, the molecular mechanisms by which CLA improves reproduction are not understood. The effect of the CLA isomers, trans‐10, cis‐12 CLA and cis‐9, trans‐11 CLA on synthesis of progesterone, PGE2, and PGF2α, in bovine luteal cells was determined in this study. Luteal cells from three cows were cultured in medium containing 0 or 0.1 µM of trans‐10, cis‐12 CLA and cis‐9, trans‐11 CLA in varying ratios in the presence and absence of 1 µM of forskolin. Prostaglandin and progesterone concentrations were not altered by CLA isomer and ratio. Luteal cells cultured in the presence of CLA had lower PGF2α concentrations (62.6 ± 13.4 pg/ml vs. 55.7 ± 12.2 pg/ml; P = 0.005) and, in the absence of forskolin, lower PGE2 concentrations (65.3 ± 15.1 pg/ml vs. 32.4 ± 14.1 pg/ml; P = 0.002) in culture media, while progesterone concentrations were not altered (P = 0.63). Relative steady‐state mRNA amounts of COX‐2 (1.7‐fold decrease; P = 0.002), PGE synthase (1.5‐fold decrease; P = 0.03) and 3β‐hydroxysteroid dehydrogenase (1.6‐fold decrease; P = 0.0003) were lower in CLA‐treated cultures, but CLA did not significantly alter mRNA amounts of PGE2 9‐keto‐reductase, StAR, and cytochrome P450 side chain cleavage enzyme. In conclusion, a potential mechanism exists by which trans‐10, cis‐12 CLA and cis‐9, trans‐11 CLA may improve reproductive performance in dairy cows, by suppressing PGF2α synthesis in luteal cells via attenuation of COX‐2 gene expression. Mol. Reprod. Dev. 78:328–336, 2011. © 2011 Wiley‐Liss, Inc.