A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [35S]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved an...

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Published inElectrophoresis Vol. 12; no. 11; p. 802
Main Authors Celis, J E, Madsen, P, Rasmussen, H H, Leffers, H, Honoré, B, Gesser, B, Dejgaard, K, Olsen, E, Magnusson, N, Kiil, J
Format Journal Article
LanguageEnglish
Published Germany 1991
Subjects
Biopsy
Cell Differentiation - physiology
Cell Division - physiology
Cells, Cultured
Databases, Factual
Electrophoresis, Gel, Two-Dimensional
Endothelium, Vascular - pathology
Humans
Immunoblotting
In Vitro Techniques
Keratinocytes - chemistry
Proteins - chemistry
Psoriasis - pathology
Reference Values
Sweat Glands - pathology
Up-Regulation - physiology
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Summary:A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [35S]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer-aided 2-D gel electrophoresis. The protein numbers in this database differ from those reported in an earlier version due to changes in the scanning hardware (Celis et al., Electrophoresis 1990, 11, 242-254). Annotation categories reported include: "protein name" (listing 207 known proteins in alphabetical order), "basal cell markers", "differentiation markers", "proteins highly up-regulated in psoriatic skin", "microsequenced proteins" and "human autoantigens". For reference, we have also included 2-D gel (isoelectric focusing) patterns of cultured normal and psoriatic keratinocytes, melanocytes, fibroblasts, dermal microvascular endothelial cells, peripheral blood mononuclear cells and sweat duct cells. The keratinocyte 2-D gel protein database will be updated yearly in the November issue of Electrophoresis.
ISSN:0173-0835
DOI:10.1002/elps.1150121105