Replication-coupled modulation of early replicating chromatin domains detected by anti-actin antibody
Evidence is presented for the reversible, cold‐dependent immunofluorescence detection of the epitope (hereafter referred to as epiC), recognized by a monoclonal anti‐actin antibody in diploid human fibroblast cell nuclei and mitotic chromosomes. The nuclear/chromosomal epiC was detected in a cell cy...
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Published in | Journal of cellular biochemistry Vol. 94; no. 5; pp. 899 - 916 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.04.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Evidence is presented for the reversible, cold‐dependent immunofluorescence detection of the epitope (hereafter referred to as epiC), recognized by a monoclonal anti‐actin antibody in diploid human fibroblast cell nuclei and mitotic chromosomes. The nuclear/chromosomal epiC was detected in a cell cycle window beginning in early S phase and extending through S phase, G2 phase, mitosis until early G1 phase of the subsequent daughter cells. A small but significant level of co‐localization was measured between the nuclear epiC and active sites of DNA replication in early S phase. The level of co‐localization was strikingly enhanced beginning approximately 1 h after the initial labeling of early S phase replicating chromatin domains. In contrast, epiC did not co‐localize with late S phase replicated chromatin either during DNA replication or at any other time in the cell cycle. We propose a replication‐coupled modulation of early S phase replicated chromatin domains that is detected by the chromatin epiC positivity, persists on the chromatin domains from early S until early G1 of the next cell generation, and may be involved in the regulation and/or coordination of replicational and transcriptional processes during the cell cycle. Further studies will be required to resolve the possible role of nuclear actin in this modulation process. J. Cell. Biochem. 94: 899–916, 2005. © 2005 Wiley‐Liss, Inc. |
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Bibliography: | Ministry of Education, Youth and Sports of the Czech Republic - No. MSM 111100003 ArticleID:JCB20374 ark:/67375/WNG-228S65Q9-2 National Institutes of Health - No. GM 072131-23 Grant Agency of the Czech Republic - No. 304/00/1622; No. 304/03/1121; No. 304/04/0692; No. 304/02/0342 Grant Agency of the Czech Academy of Sciences - No. IAA5039103; No. AV0Z5039906 istex:0DE150069B5DDDE1510FF45D38BDEAC07E5D58DB ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0730-2312 1097-4644 |
DOI: | 10.1002/jcb.20374 |