Principal component analysis and artificial neural network analysis of oral tissue fluorescence spectra: Classification of normal premalignant and malignant pathological conditions

• Published in: Biopolymers Vol. 82; no. 2; pp. 152 - 166
• Main Authors: Nayak, G. S., Kamath, Sudha, Pai, Keerthilatha M., Sarkar, Arindam, Ray, Satadru, Kurien, Jacob, D'Almeida, Lawrence, Krishnanand, B. R., Santhosh, C., Kartha, V. B., Mahato, K. K.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05.06.2006
• Subjects: Aged; artificial neural network; Computer Simulation; Fluorescence; Humans; laser-induced fluorescence; Middle Aged; Mouth Neoplasms - classification; Mouth Neoplasms - pathology; Neural Networks (Computer); oral tissue; Precancerous Conditions - classification; Precancerous Conditions - pathology; Principal Component Analysis; Spectrometry, Fluorescence
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/bip.20473

Pulsed laser‐induced autofluorescence spectroscopic studies of pathologically certified normal, premalignant, and malignant oral tissues were carried out at 325 nm excitation. The spectral analysis and classification for discrimination among normal, premalignant, and malignant conditions were performed using principal component analysis (PCA) and artificial neural network (ANN) separately on the same set of spectral data. In case of PCA, spectral residuals, Mahalanobis distance, and scores of factors were used for discrimination among normal, premalignant, and malignant cases. In ANN, parameters like mean, spectral residual, standard deviation, and total energy were used to train the network. The ANN used in this study is a classical multiplayer feed‐forward type with a back‐propagation algorithm for the training of the network. The specificity and sensitivity were determined in both classification schemes. In the case of PCA, they are 100 and 92.9%, respectively, whereas for ANN they are 100 and 96.5% for the data set considered. © 2006 Wiley Periodicals, Inc. Biopolymers 82: 152–166, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com