Simultaneous generation of fra-2 conditional and fra-2 knock-out mice
Loss of function mouse models comprise knock‐out mice, where a gene is deleted in the germline, and conditional knock‐out mice with somatic deletion of a floxed allele in defined tissues. Both types of mice are used for comprehensive studies of gene functions in vivo. Here, we describe a simple meth...
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Published in | Genesis (New York, N.Y. : 2000) Vol. 45; no. 7; pp. 447 - 451 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.07.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Loss of function mouse models comprise knock‐out mice, where a gene is deleted in the germline, and conditional knock‐out mice with somatic deletion of a floxed allele in defined tissues. Both types of mice are used for comprehensive studies of gene functions in vivo. Here, we describe a simple method for simultaneous generation of mice with conditional or knock‐out alleles for the transcription factor fra‐2 (Fos‐related antigen 2) using a single embryonic stem (ES) cell clone. ES cells with a floxed fra‐2 allele were transiently transfected with a Cre‐recombinase expression plasmid and plated at low density. Most of the resulting ES cell colonies consisted of a mixture of cells that have either retained or lost the conditional allele. We demonstrate that these mixed ES cell clones can be directly used for generation of chimeras that give rise to offspring with conditional or knock‐out alleles simultaneously. This strategy shortens the time and reduces the number of germline transmission events to generate genetically modified mice. genesis 45:447–451, 2007. © 2007 Wiley‐Liss, Inc. |
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Bibliography: | istex:721E1BFF78581C531464CEAFD6477D3362CDE576 ArticleID:DVG20311 ark:/67375/WNG-X2Q9MH6T-T Boehringer Ingelheim Ludwig Boltzmann Gesellschaft Austrian Industrial Research Promotion Fund FWF - No. SFB-F28 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1526-954X 1526-968X |
DOI: | 10.1002/dvg.20311 |