Determination of the Apparent Michaelis Constant of Glucose Oxidase Immobilized on a Microelectrode with Respect to Oxygen

We report determination of the apparent Michaelis constant of glucose oxidase (GOx) immobilized on a microelectrode with respect to oxygen. We used a GOx‐modified microelectrode as a probe for scanning electrochemical microscopy. We detected hydrogen peroxide generated by the enzyme reaction at the...

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Published inElectroanalysis (New York, N.Y.) Vol. 22; no. 9; pp. 927 - 930
Main Authors Yasukawa, Tomoyuki, Goto, Keisuke, Mizutani, Fumio
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.05.2010
WILEY‐VCH Verlag
Subjects
Apparent Michaelis constant
Arrays
Electrodes
Electrolysis
Glucose oxidase
Hydrogen peroxide
Microelectrode
Microelectrodes
Microscopy
Microsensors
Oxygen
Scanning
Scanning electrochemical microscopy
Sensors
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Summary:We report determination of the apparent Michaelis constant of glucose oxidase (GOx) immobilized on a microelectrode with respect to oxygen. We used a GOx‐modified microelectrode as a probe for scanning electrochemical microscopy. We detected hydrogen peroxide generated by the enzyme reaction at the microelectrode under controlling the oxygen concentration using water electrolysis at an interdigitated array (IDA) electrode. The response depends on the oxygen concentration, which is regulated by the microelectrode position and the potential applied to the IDA electrode. We estimated the apparent Michaelis constant with respect to oxygen in this experimental condition to be about 0.28 mM.
Bibliography:istex:6E0394741F4E136CFB5997D0FE88DB707F1D1855
ark:/67375/WNG-FW3RL7ZQ-1
ArticleID:ELAN200900501
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
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ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200900501