Effect of PD fluid instillation on the peritonitis‐induced influx and bacterial clearing capacity of peritoneal cells

• Published in: Nephrology, dialysis, transplantation Vol. 16; no. 3; pp. 679 - 682
• Main Authors: Hekking, Liesbeth H. P., Huijsmans, Agnes, Van Gelderop, Erwin, Wieslander, Andes P., Havenith, Carin E. G., van den Born, Jacob, Beelen, Robert H. J.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Oxford University Press 01.03.2001
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Bacteria - immunology; Biological and medical sciences; Dialysis Solutions - pharmacology; Emergency and intensive care: renal failure. Dialysis management; Epithelium - drug effects; Epithelium - immunology; Epithelium - pathology; Intensive care medicine; Male; Medical sciences; mesothelial cells; Peritoneal Dialysis; peritoneal dialysis fluid; peritoneal immune defence; Peritoneum - drug effects; Peritoneum - immunology; Peritoneum - pathology; Peritonitis - immunology; Peritonitis - microbiology; rat model; Rats; Rats, Wistar; Extrarenal dialysis; Animal model; Vertebrata; Mammalia; Rat; Animal; Rodentia; Dialysis fluid; Glucose; Peritoneal dialysis; Comparative study
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/16.3.679

Background. The commonly used peritoneal dialysis fluids contain glucose as the osmotic agent. Heat sterilization leads to the formation of glucose degradation products which contribute, together with glucose, to the formation of advanced glycation end‐products (AGEs). AGEs have been shown to be present in the peritoneal cavity. Methods have been developed to minimize the amount of glucose degradation products in peritoneal dialysis fluids. In a rat peritoneal dialysis model, we compare the effect of a commonly used peritoneal dialysis fluid, Gambrosol®, with a newly developed peritoneal dialysis fluid, PD‐Bio®, on the influx and functional capacity of the peritoneal cells after 2 weeks of peritoneal dialysis fluid instillation. Methods. Three groups of animals were used: rats received daily infusion with 15 ml of either 4% Gambrosol® (group 1) or 4% PD‐Bio® (group 2), and a control group of animals did not receive fluid (group 3). After 2 weeks of PD fluid instillation, all the animals were injected with a 0.5 ml suspension containing 3×108 colony‐forming units of Staphylococcus aureus. The in vivo bacterial clearing capacity was determined after 15 h. Results. A statistically significant higher leukocyte influx was found in the control group compared with both PD fluid‐injected groups. No statistical differences in bacterial clearing were observed among the three groups, although the number of bacteria recovered from the PD‐Bio® group tended to be lower than that from the Gambrosol® group. Moreover, in both PD fluid instillation groups, the bacteria tended to be cleared more slowly compared with the control group. The number of mesothelial cells in the PD fluid groups was significantly greater than in the control group. Conclusion. No differences were observed in bacterial clearing capacity, leukocyte influx and mesothelial cell number after a 2 week exposure of the peritoneal cavity to Gambrosol®vs PD‐Bio®.