Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp

• Published in: Journal of biotechnology Vol. 60; no. 1-2; pp. 113 - 117
• Main Authors: Mohapatra, B.R, Banerjee, U.C, Bapuji, M
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 05.02.1998; Amsterdam Elsevier; New York, NY
• Subjects: Activation and deactivation energies; Amylase; aquatic organisms; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Fundamental and applied biological sciences. Psychology; Fungi; Marine; Miscellaneous; Mission oriented research; Mucor; Mucor sp; plant biochemistry; plant physiology; Spirastrella; Spirastrella sp; Thermostability; Thermostability; Spirastrella sp; Activation and deactivation energies; Amylase; Mucor sp; Enzyme; Phycomycetes; Environmental factor; Mucor; Thermal stability; Fungi; Characterization; Marine environment; Enzymatic activity; Porifera; Isolation; Invertebrata; Thallophyta
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/S0168-1656(97)00197-1

A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation and deactivation energies of the partially purified enzyme were 46.60 and 157.05 kJ mol−1, respectively. The enzyme activity was not affected by the addition of 3% NaCl, 10 mM Ca2+ and 25 mM Mg2+, but was strongly inhibited by EDTA.