Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp
A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation...
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Published in | Journal of biotechnology Vol. 60; no. 1-2; pp. 113 - 117 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
05.02.1998
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation and deactivation energies of the partially purified enzyme were 46.60 and 157.05 kJ mol−1, respectively. The enzyme activity was not affected by the addition of 3% NaCl, 10 mM Ca2+ and 25 mM Mg2+, but was strongly inhibited by EDTA. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0168-1656 1873-4863 |
DOI: | 10.1016/S0168-1656(97)00197-1 |