Genomic description and characterization of Nigeribacterium massiliense gen. nov., sp. nov., isolated from the human gut

• Published in: Microbes and infection Vol. 23; no. 8; p. 104842
• Main Authors: Khare, Mudra, Sinha, Dhiraj, Zgheib, Rita, Traore, Sory Ibrahima, Lagier, Jean-Christophe, Raoult, Didier, Fournier, Pierre-Edouard
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.09.2021; Elsevier
• Subjects: Bacteriology; Base Composition; Cardiology and cardiovascular system; Child, Preschool; Culturomics; DNA, Bacterial - genetics; Emerging diseases; Genomics; Human health and pathology; Humans; Infectious diseases; Life Sciences; Male; Microbiology and Parasitology; Nigeribacterium massiliense; Parasitology; Phylogeny; RNA, Ribosomal, 16S - genetics; Taxono-genomics; Taxonomy; Virology; Taxono-genomics; Taxonomy; Genomics; Culturomics; Nigeribacterium massiliense
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/j.micinf.2021.104842

Strain Marseille-P1302 was isolated from the stool of a 2-year-old Nigerian boy suffering from Kwashiorkor, a form of severe acute malnutrition. The strain grows in aerobic atmosphere and bacterial cells are Gram-positive cocci ranging in diameter from 0.8 to 1 μm. Among species with standing in nomenclature, strain Marseille-P1302 exhibited a highest 16S rRNA sequence similarity of 94.97% with Brevilactibacter flavus strain VG341T, but was phylogenetically-closest to Brevilactibacter sinopodophylli strains KCTC 33808T. The draft genome of strain Marseille-P1302 was 2,934,258-bp-long with a 70.38% G + C content, and contained 2704 protein-coding genes and 55 RNAs that included 9 rRNA genes. On the basis of these data, we propose the creation of the new genus Nigeribacterium gen. nov., with strain Marseille-P1302T (= CSUR P1302 = DSM 29084) being the type strain of the new species Nigeribacterium. massiliense gen. nov., sp. nov.