Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives

• Published in: IOP conference series. Earth and environmental science Vol. 101; no. 1; pp. 12033 - 12040
• Main Authors: Karimy, M F, Sutrisno, B, Agus, A, Suryani, A E, Istiqomah, L, Damayanti, E
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.12.2017
• Subjects: Additives; Aflatoxicosis; Aflatoxins; Bursa of Fabricius; Chronic exposure; Contamination; Corn; Diet; Erythrocytes; Histopathology; Low level; Methionine; Variance analysis
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/101/1/012033

The aim of this study was to evaluate erythrocyte profile and microscopic changes effect of AF induces by low level (57.18 ppb) and chronic exposure (34 days) with administration of additive (Lactobacillus plantarum G7 and methionine). Aflatoxin-contaminated corn was prepared by inoculate Aspergillus flavus FNCC 6002 on corn. Total number of 576 broiler Lohman strain (MB202) unsexed DOC were allocated completely randomized into four treatments and 12 replicates, with 12 broiler chicks each. The treatments as follows: T1 = aflatoxin-contaminated diet, T2 = aflatoxin-contaminated diet + 1% of LAB (w/w), T3 = aflatoxin-contaminated diet + 0.8% of methionine (w/w), and T4 = aflatoxin-contaminated diet + 1% of LAB + 0.8% of methionine (w/w). The effect of treatments was evaluated using ANOVA and the difference among mean treatments were analyzed using DMRT. The result showed that administration of additives had no significant effect (P>0.05) on erythrocyte profile, liver, and bursa of Fabricius. The dose of additive in each treatment (T2, T3, T4) were insufficient to reduce adverse effect of chronic aflatoxicosis. It was concluded that the LAB dose for binding AF (57.18%) should be evaluated and the dose for methionine should be reduced for chronic treatment of aflatoxicosis.