LC-APCI-MS/MS analysis of urinary 8-hydroxy-2′-deoxyguanosine

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 32; no. 4; pp. 657 - 661
• Main Authors: Pietta, P.G., Simonetti, P., Gardana, C., Cristoni, S., Bramati, L., Mauri, P.L.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 08.08.2003; Elsevier Science
• Subjects: 8OHdG; Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; APCI; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid - methods; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - chemistry; Deoxyguanosine - urine; Fundamental and applied biological sciences. Psychology; Humans; LC-MS; Mass Spectrometry - methods; MRM; Spectrometry, Mass, Electrospray Ionization - methods; Urine; Urine; MRM; LC-MS; APCI; 8OHdG; Human; Biological fluid; Purine nucleotide; Purine derivatives; Guanosine; Liquid chromatography; Chemical ionization; Hydrolysis; Ion trap; Spectrometry; Analysis method; Mass spectrometry MS/MS; Oxidation; Derived product

8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, it...