LC-APCI-MS/MS analysis of urinary 8-hydroxy-2′-deoxyguanosine

8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, it...

Full description

Saved in:
Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 32; no. 4; pp. 657 - 661
Main Authors Pietta, P.G., Simonetti, P., Gardana, C., Cristoni, S., Bramati, L., Mauri, P.L.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 08.08.2003
Elsevier Science
Subjects
8OHdG
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
APCI
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid - methods
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - chemistry
Deoxyguanosine - urine
Fundamental and applied biological sciences. Psychology
Humans
LC-MS
Mass Spectrometry - methods
MRM
Spectrometry, Mass, Electrospray Ionization - methods
Urine
Urine
MRM
LC-MS
APCI
8OHdG
Human
Biological fluid
Purine nucleotide
Purine derivatives
Guanosine
Liquid chromatography
Chemical ionization
Hydrolysis
Ion trap
Spectrometry
Analysis method
Mass spectrometry MS/MS
Oxidation
Derived product
Online AccessGet full text

Cover

More Information
Summary:8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, its hydrolysis and possible derivatization (as for GC-MS), invalidating the measurement of 8OHdG. Therefore, the direct analysis of 8OHdG excreted into urine was preferred. Interferences from the urine matrix were excluded by applying LC-APCI-MS/MS in the multiple reaction monitoring (MRM) mode. The abundant fragment ion at m/z 168 arising from 8OHdG was monitored in the urine sample of volunteers supplemented with tomato concentrate for different times. The procedure allowed the detection of levels of 8OHdG as low as 1 ng/ml in urine sample.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00172-9