LC-APCI-MS/MS analysis of urinary 8-hydroxy-2′-deoxyguanosine

8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, it...

Full description

Saved in:
Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 32; no. 4; pp. 657 - 661
Main Authors Pietta, P.G., Simonetti, P., Gardana, C., Cristoni, S., Bramati, L., Mauri, P.L.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 08.08.2003
Elsevier Science
Subjects
8OHdG
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
APCI
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid - methods
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - chemistry
Deoxyguanosine - urine
Fundamental and applied biological sciences. Psychology
Humans
LC-MS
Mass Spectrometry - methods
MRM
Spectrometry, Mass, Electrospray Ionization - methods
Urine
Urine
MRM
LC-MS
APCI
8OHdG
Human
Biological fluid
Purine nucleotide
Purine derivatives
Guanosine
Liquid chromatography
Chemical ionization
Hydrolysis
Ion trap
Spectrometry
Analysis method
Mass spectrometry MS/MS
Oxidation
Derived product
Online AccessGet full text

Cover

Abstract 8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, its hydrolysis and possible derivatization (as for GC-MS), invalidating the measurement of 8OHdG. Therefore, the direct analysis of 8OHdG excreted into urine was preferred. Interferences from the urine matrix were excluded by applying LC-APCI-MS/MS in the multiple reaction monitoring (MRM) mode. The abundant fragment ion at m/z 168 arising from 8OHdG was monitored in the urine sample of volunteers supplemented with tomato concentrate for different times. The procedure allowed the detection of levels of 8OHdG as low as 1 ng/ml in urine sample.
AbstractList 8-Hydroxy-2'-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, its hydrolysis and possible derivatization (as for GC-MS), invalidating the measurement of 8OHdG. Therefore, the direct analysis of 8OHdG excreted into urine was preferred. Interferences from the urine matrix were excluded by applying LC-APCI-MS/MS in the multiple reaction monitoring (MRM) mode. The abundant fragment ion at m/z 168 arising from 8OHdG was monitored in the urine sample of volunteers supplemented with tomato concentrate for different times. The procedure allowed the detection of levels of 8OHdG as low as 1 ng/ml in urine sample.
8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in various biological matrices, most notably DNA and urine. In the case of DNA, artifactual oxidation may take place during the isolation of DNA, its hydrolysis and possible derivatization (as for GC-MS), invalidating the measurement of 8OHdG. Therefore, the direct analysis of 8OHdG excreted into urine was preferred. Interferences from the urine matrix were excluded by applying LC-APCI-MS/MS in the multiple reaction monitoring (MRM) mode. The abundant fragment ion at m/z 168 arising from 8OHdG was monitored in the urine sample of volunteers supplemented with tomato concentrate for different times. The procedure allowed the detection of levels of 8OHdG as low as 1 ng/ml in urine sample.
Author Pietta, P.G.
Mauri, P.L.
Gardana, C.
Simonetti, P.
Bramati, L.
Cristoni, S.
Author_xml – sequence: 1
  givenname: P.G.
  surname: Pietta
  fullname: Pietta, P.G.
  email: pietta@itba.mi.cnr.it
  organization: CNR, Istituto di Tecnologie Biomediche (ITB), Via F.lli Cervi 93, 20090 Segrate, Milano, Italy
– sequence: 2
  givenname: P.
  surname: Simonetti
  fullname: Simonetti, P.
  organization: Università degli Studi di Milano, diSTAM, Sez. Nutrizione, Via Celoria 2, 20133 Milano, Italy
– sequence: 3
  givenname: C.
  surname: Gardana
  fullname: Gardana, C.
  organization: Università degli Studi di Milano, diSTAM, Sez. Nutrizione, Via Celoria 2, 20133 Milano, Italy
– sequence: 4
  givenname: S.
  surname: Cristoni
  fullname: Cristoni, S.
  organization: CNR, Istituto di Tecnologie Biomediche (ITB), Via F.lli Cervi 93, 20090 Segrate, Milano, Italy
– sequence: 5
  givenname: L.
  surname: Bramati
  fullname: Bramati, L.
  organization: CNR, Istituto di Tecnologie Biomediche (ITB), Via F.lli Cervi 93, 20090 Segrate, Milano, Italy
– sequence: 6
  givenname: P.L.
  surname: Mauri
  fullname: Mauri, P.L.
  organization: CNR, Istituto di Tecnologie Biomediche (ITB), Via F.lli Cervi 93, 20090 Segrate, Milano, Italy
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15055145$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/12899955$$D View this record in MEDLINE/PubMed
BookMark eNqFkMtKAzEUhoMoWi-PoHSj6CJ6MpnMJCuR4g0qClVwF2LmjEamGU06Ync-k4_kk5jaoktX5yy-_1y-dbLsW4-EbDM4ZMCKoxGUnNESpNgHfgDAyoyqJdJjsuQ0K_L7ZdL7RdbIeozPACCYylfJGsukUkqIHjkeDujJzeCSXo2OrkZ9400zjS7227rfBedNmPYlfZpWoX2f0uzr45NWmNrHzvg2Oo-bZKU2TcStRd0gd2ent4MLOrw-vxycDKnlik0oMgsina0ypYqap9XcVIBcgeGqZsJwUTKEXPLCVFLkuXzIhMxYUeTC2gRukL353JfQvnYYJ3rsosWmMR7bLuqSi1xmXCRQzEEb2hgD1voluHH6QzPQM3P6x5yeadHA9Y85rVJuZ7Ggexhj9ZdaqErA7gIw0ZqmDsZbF_84AUKwfMYdzzlMOt4cBh2tQ2-xcgHtRFet--eUb0o0icM
CODEN JPBADA
CitedBy_id crossref_primary_10_1002_jms_594
crossref_primary_10_1002_jssc_200600314
crossref_primary_10_1134_S1061934808010103
crossref_primary_10_1016_j_cca_2009_12_013
crossref_primary_10_1088_1757_899X_188_1_012010
crossref_primary_10_1081_JLC_120030603
crossref_primary_10_1016_j_jchromb_2006_11_033
crossref_primary_10_1002_elan_200804324
crossref_primary_10_1007_s11095_009_9865_7
crossref_primary_10_1007_s00216_010_4217_3
crossref_primary_10_1002_rcm_5321
crossref_primary_10_1016_j_jchromb_2011_10_026
crossref_primary_10_1002_rcm_3299
crossref_primary_10_1002_rcm_1763
Cites_doi 10.1111/j.1753-4887.1997.tb06100.x
10.1080/10715769800300531
10.1016/S0891-5849(01)00462-2
10.1093/ajcn/72.5.1082
10.1080/10715760000301411
10.1016/0959-8049(94)00490-0
10.1080/10715769800300651
10.1093/ajcn/69.4.712
10.1016/S1383-5742(97)00035-5
10.1002/(SICI)1097-0231(19990530)13:10<924::AID-RCM588>3.0.CO;2-G
10.1021/ac000866n
ContentType Journal Article
Conference Proceeding
Copyright 2003 Elsevier Science B.V.
2003 INIST-CNRS
Copyright_xml – notice: 2003 Elsevier Science B.V.
– notice: 2003 INIST-CNRS
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1016/S0731-7085(03)00172-9
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Pharmacy, Therapeutics, & Pharmacology
EISSN 1873-264X
EndPage 661
ExternalDocumentID 10_1016_S0731_7085_03_00172_9
12899955
15055145
S0731708503001729
Genre Journal Article
GroupedDBID ---
--K
--M
.~1
0R~
1B1
1RT
1~.
1~5
29L
4.4
457
4G.
53G
5GY
5VS
7-5
71M
8P~
9JM
9JN
AABNK
AACTN
AAEDT
AAEDW
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AARLI
AATCM
AAXUO
ABFNM
ABFRF
ABJNI
ABMAC
ABXDB
ABYKQ
ABZDS
ACDAQ
ACGFO
ACGFS
ACIUM
ACNNM
ACRLP
ADBBV
ADECG
ADEZE
ADMUD
AEBSH
AEFWE
AEKER
AENEX
AFKWA
AFTJW
AFXIZ
AFZHZ
AGHFR
AGUBO
AGYEJ
AHHHB
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
AJQLL
AJSZI
ALCLG
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
C45
CS3
DU5
EBS
EFJIC
EFLBG
EJD
EO8
EO9
EP2
EP3
F5P
FDB
FEDTE
FGOYB
FIRID
FLBIZ
FNPLU
FYGXN
G-2
G-Q
GBLVA
HMT
HMU
HVGLF
HZ~
IHE
J1W
KOM
M34
M36
M41
MO0
N9A
O-L
O9-
OAUVE
OGGZJ
OVD
OZT
P-8
P-9
P2P
PC.
Q38
R2-
RIG
RNS
ROL
RPZ
SCB
SCC
SCH
SDF
SDG
SDP
SES
SEW
SPC
SPCBC
SPT
SSK
SSP
SSZ
T5K
TEORI
WUQ
XFK
XPP
YK3
~G-
ABPIF
ABPTK
IQODW
AAXKI
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
AFJKZ
CITATION
7X8
ID FETCH-LOGICAL-c391t-e1c0501692996f39553ad0e390a39f15a3571e04836ad85448b258216645cc0e3
IEDL.DBID .~1
ISSN 0731-7085
IngestDate Thu Oct 24 23:17:05 EDT 2024
Thu Sep 26 15:47:35 EDT 2024
Sat Sep 28 08:38:10 EDT 2024
Sun Oct 22 16:05:12 EDT 2023
Fri Feb 23 02:31:11 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords Urine
MRM
LC-MS
APCI
8OHdG
Human
Biological fluid
Purine nucleotide
Purine derivatives
Guanosine
Liquid chromatography
Chemical ionization
Hydrolysis
Ion trap
Spectrometry
Analysis method
Mass spectrometry MS/MS
Oxidation
Derived product
Language English
License CC BY 4.0
LinkModel DirectLink
MeetingName Drug Analysis 2002, Bruges, Belgium, 21-24 April, 2002
MergedId FETCHMERGED-LOGICAL-c391t-e1c0501692996f39553ad0e390a39f15a3571e04836ad85448b258216645cc0e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 12899955
PQID 73548235
PQPubID 23479
PageCount 5
ParticipantIDs proquest_miscellaneous_73548235
crossref_primary_10_1016_S0731_7085_03_00172_9
pubmed_primary_12899955
pascalfrancis_primary_15055145
elsevier_sciencedirect_doi_10_1016_S0731_7085_03_00172_9
PublicationCentury 2000
PublicationDate 2003-08-08
PublicationDateYYYYMMDD 2003-08-08
PublicationDate_xml – month: 08
  year: 2003
  text: 2003-08-08
  day: 08
PublicationDecade 2000
PublicationPlace Amsterdam
PublicationPlace_xml – name: Amsterdam
– name: England
PublicationTitle Journal of pharmaceutical and biomedical analysis
PublicationTitleAlternate J Pharm Biomed Anal
PublicationYear 2003
Publisher Elsevier B.V
Elsevier Science
Publisher_xml – name: Elsevier B.V
– name: Elsevier Science
References Kasai (BIB6) 1997; 387
Lunec (BIB7) 1998; 29
Roberts, Churchwell, Beland, Fang, Doerge (BIB5) 2001; 73
H. Sies (Ed.), Oxidative Stress, Academic Press, London, 1985.
Duez, Helson, Some, Dubois, Hanocq (BIB4) 2000; 33
Mauri, Iemoli, Gardana, Riso, Simonetti, Porrini, Pietta (BIB10) 1999; 13
Halliwell (BIB8) 2000; 72
Tagesson, Kallberg, Klintenberg, Starkhammar (BIB11) 1995; 31A
Riso, Pinder, Santangelo, Porrini (BIB12) 1999; 69
Halliwell (BIB2) 1997; 55
Poulsen, Loft, Weimann (BIB13) 2000
Halliwell (BIB3) 1998; 29
Weimann, Belling, Poulsen (BIB9) 2001; 30
Tagesson (10.1016/S0731-7085(03)00172-9_BIB11) 1995; 31A
Kasai (10.1016/S0731-7085(03)00172-9_BIB6) 1997; 387
10.1016/S0731-7085(03)00172-9_BIB1
Riso (10.1016/S0731-7085(03)00172-9_BIB12) 1999; 69
Roberts (10.1016/S0731-7085(03)00172-9_BIB5) 2001; 73
Weimann (10.1016/S0731-7085(03)00172-9_BIB9) 2001; 30
Duez (10.1016/S0731-7085(03)00172-9_BIB4) 2000; 33
Halliwell (10.1016/S0731-7085(03)00172-9_BIB8) 2000; 72
Halliwell (10.1016/S0731-7085(03)00172-9_BIB2) 1997; 55
Halliwell (10.1016/S0731-7085(03)00172-9_BIB3) 1998; 29
Lunec (10.1016/S0731-7085(03)00172-9_BIB7) 1998; 29
Mauri (10.1016/S0731-7085(03)00172-9_BIB10) 1999; 13
Poulsen (10.1016/S0731-7085(03)00172-9_BIB13) 2000
References_xml – volume: 29
  start-page: 469
  year: 1998
  end-page: 486
  ident: BIB3
  publication-title: Free Rad. Res.
  contributor:
    fullname: Halliwell
– volume: 72
  start-page: 1082
  year: 2000
  end-page: 1087
  ident: BIB8
  publication-title: Am. J. Clin. Nutr.
  contributor:
    fullname: Halliwell
– volume: 69
  start-page: 712
  year: 1999
  end-page: 718
  ident: BIB12
  publication-title: Am. J. Clin. Nutr.
  contributor:
    fullname: Porrini
– volume: 29
  start-page: 601
  year: 1998
  end-page: 608
  ident: BIB7
  article-title: ESCODD: European Standards Committee on Oxidative DNA Damage
  publication-title: Free Rad. Res.
  contributor:
    fullname: Lunec
– volume: 387
  start-page: 147
  year: 1997
  end-page: 163
  ident: BIB6
  publication-title: Mutat. Res.
  contributor:
    fullname: Kasai
– volume: 30
  start-page: 757
  year: 2001
  end-page: 764
  ident: BIB9
  publication-title: Free Rad. Biol. Med.
  contributor:
    fullname: Poulsen
– volume: 13
  start-page: 924
  year: 1999
  end-page: 931
  ident: BIB10
  publication-title: Rapid Comm. Mass. Spectrom.
  contributor:
    fullname: Pietta
– volume: 55
  start-page: S44
  year: 1997
  end-page: S49
  ident: BIB2
  publication-title: Nutr. Rev.
  contributor:
    fullname: Halliwell
– volume: 73
  start-page: 303
  year: 2001
  end-page: 309
  ident: BIB5
  publication-title: Anal. Chem.
  contributor:
    fullname: Doerge
– volume: 31A
  start-page: 934
  year: 1995
  end-page: 940
  ident: BIB11
  publication-title: Eur. J. Cancer
  contributor:
    fullname: Starkhammar
– volume: 33
  start-page: 243
  year: 2000
  end-page: 260
  ident: BIB4
  publication-title: Free Rad. Res.
  contributor:
    fullname: Hanocq
– year: 2000
  ident: BIB13
  publication-title: Oxidative Damage: A Practical Approach
  contributor:
    fullname: Weimann
– volume: 55
  start-page: S44
  year: 1997
  ident: 10.1016/S0731-7085(03)00172-9_BIB2
  publication-title: Nutr. Rev.
  doi: 10.1111/j.1753-4887.1997.tb06100.x
  contributor:
    fullname: Halliwell
– volume: 29
  start-page: 469
  year: 1998
  ident: 10.1016/S0731-7085(03)00172-9_BIB3
  publication-title: Free Rad. Res.
  doi: 10.1080/10715769800300531
  contributor:
    fullname: Halliwell
– volume: 30
  start-page: 757
  year: 2001
  ident: 10.1016/S0731-7085(03)00172-9_BIB9
  publication-title: Free Rad. Biol. Med.
  doi: 10.1016/S0891-5849(01)00462-2
  contributor:
    fullname: Weimann
– volume: 72
  start-page: 1082
  year: 2000
  ident: 10.1016/S0731-7085(03)00172-9_BIB8
  publication-title: Am. J. Clin. Nutr.
  doi: 10.1093/ajcn/72.5.1082
  contributor:
    fullname: Halliwell
– volume: 33
  start-page: 243
  year: 2000
  ident: 10.1016/S0731-7085(03)00172-9_BIB4
  publication-title: Free Rad. Res.
  doi: 10.1080/10715760000301411
  contributor:
    fullname: Duez
– volume: 31A
  start-page: 934
  year: 1995
  ident: 10.1016/S0731-7085(03)00172-9_BIB11
  publication-title: Eur. J. Cancer
  doi: 10.1016/0959-8049(94)00490-0
  contributor:
    fullname: Tagesson
– year: 2000
  ident: 10.1016/S0731-7085(03)00172-9_BIB13
  contributor:
    fullname: Poulsen
– volume: 29
  start-page: 601
  year: 1998
  ident: 10.1016/S0731-7085(03)00172-9_BIB7
  article-title: ESCODD: European Standards Committee on Oxidative DNA Damage
  publication-title: Free Rad. Res.
  doi: 10.1080/10715769800300651
  contributor:
    fullname: Lunec
– volume: 69
  start-page: 712
  year: 1999
  ident: 10.1016/S0731-7085(03)00172-9_BIB12
  publication-title: Am. J. Clin. Nutr.
  doi: 10.1093/ajcn/69.4.712
  contributor:
    fullname: Riso
– volume: 387
  start-page: 147
  year: 1997
  ident: 10.1016/S0731-7085(03)00172-9_BIB6
  publication-title: Mutat. Res.
  doi: 10.1016/S1383-5742(97)00035-5
  contributor:
    fullname: Kasai
– volume: 13
  start-page: 924
  year: 1999
  ident: 10.1016/S0731-7085(03)00172-9_BIB10
  publication-title: Rapid Comm. Mass. Spectrom.
  doi: 10.1002/(SICI)1097-0231(19990530)13:10<924::AID-RCM588>3.0.CO;2-G
  contributor:
    fullname: Mauri
– ident: 10.1016/S0731-7085(03)00172-9_BIB1
– volume: 73
  start-page: 303
  year: 2001
  ident: 10.1016/S0731-7085(03)00172-9_BIB5
  publication-title: Anal. Chem.
  doi: 10.1021/ac000866n
  contributor:
    fullname: Roberts
SSID ssj0005194
Score 1.8373013
Snippet 8-Hydroxy-2′-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in...
8-Hydroxy-2'-deoxyguanosine (8OHdG) is regarded as an important biomarker of oxidative DNA damage and it may be estimated by using different techniques in...
SourceID proquest
crossref
pubmed
pascalfrancis
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 657
SubjectTerms 8OHdG
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
APCI
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid - methods
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - chemistry
Deoxyguanosine - urine
Fundamental and applied biological sciences. Psychology
Humans
LC-MS
Mass Spectrometry - methods
MRM
Spectrometry, Mass, Electrospray Ionization - methods
Urine
Title LC-APCI-MS/MS analysis of urinary 8-hydroxy-2′-deoxyguanosine
URI https://dx.doi.org/10.1016/S0731-7085(03)00172-9
https://www.ncbi.nlm.nih.gov/pubmed/12899955
https://search.proquest.com/docview/73548235
Volume 32
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NThsxELYQXCpVFfQ3BVIfKtRKmHjjtTc-oSgqCm2DIgUkbpbXa1Mum4gkh1wQz8Qj8STMeDfackBI3FbW2Gt9nh3PeGc-E_LdBYWEAIJ5ZRVLZeBMKx9YlkOEKwXPXcAC59GZGl6kvy_l5QYZrGthMK2ytv2VTY_Wum7p1Gh2ZtfXnQkoZ5Ih45qIgQwW8SHZFuj00e1_aR5JvAwRhRlKN1U81Qix8QcXP-MgTD-3P72d2TmgFqrrLp73R-O-dLJN3tUOJe1Xc94hG758Tw7GFSP16pCeNwVW80N6QMcNV_XqAzn-O2D98eCUjSad0YTamqKETgPFY3h7s6I99m9VYLIL6z7c3bPCw-PV0pZTzJj_SC5Ofp0Phqy-U4E5oZMF84njEhlYYBtSQWgphS24F5pboUMirZBZ4pFnXtmiJyF4y7tYS6tUKp0DwU9ks5yW_guhTueZdjwX4DSlmhe5EGnXZYVWNguF9S1ytEbSzCrqDNPklAH0BqE3XJgIvdEt0lvjbZ7ogAHz_lLX9pP1aV4IHh74hLJFvq0XzMAHhH9FbOmny7nJBARtXQESn6t1bPpiMAoIfX39vHbJm5j9hzkmvT2yubhZ-n3wYhZ5O6ppm2z1T_8Mzx4BSN_pnw
link.rule.ids 310,311,315,783,787,792,793,4511,23944,23945,24130,25154,27938,27939,45599,45693
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3BahsxEBVpckihhKZpUzdtokMJLUSx1lpprVMJpsFp7WCwA7kJrVZKclmb2D74UvJN_aR-SWa0a7Y5hEJvixhpxdPsaEY780TIZxcUEgII5pVVLJWBM618YFkOEa4UPHcBC5yHl6p_lf64ltcbpLeuhcG0ytr2VzY9Wuu6pV2j2Z7d3bXHoJxJhoxrIgYy-gXZSpE_C5T69NdfeR5JvA0RpRmKN2U81RCx8QsXX-MoTD-3Qb2a2TnAFqr7Lp53SOPGdP6a7NQeJT2rJr1LNnz5hhyPKkrq1QmdNBVW8xN6TEcNWfVqj3wb9NjZqHfBhuP2cExtzVFCp4HiOby9X9Euu10VmO3COn8efrPCw-PN0pZTTJl_S67Ov096fVZfqsCc0MmC-cRxiRQssA-pILSUwhbcC82t0CGRVsgs8Ug0r2zRlRC95R0splUqlc6B4DuyWU5L_55Qp_NMO54L8JpSzYtciLTjskIrm4XC-hY5XSNpZhV3hmmSygB6g9AbLkyE3ugW6a7xNk-UwIB9_1fXwyfr07wQXDxwCmWLHK0XzMAXhL9FbOmny7nJBERtHQES-9U6Nn0xGgWEPvz_vI7Idn8yHJjBxeXPA_IypgJiwkn3I9lc3C_9J3BpFvlhVNlH55vrQQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=proceeding&rft.title=Journal+of+pharmaceutical+and+biomedical+analysis&rft.atitle=LC-APCI-MS%2FMS+analysis+of+urinary+8-hydroxy-2%27-deoxyguanosine&rft.au=PIETTA%2C+P.+G&rft.au=SIMONETTI%2C+P&rft.au=GARDANA%2C+C&rft.au=CRISTONI%2C+S&rft.date=2003-08-08&rft.pub=Elsevier+Science&rft.issn=0731-7085&rft.eissn=1873-264X&rft.volume=32&rft.issue=4-5&rft.spage=657&rft.epage=661&rft_id=info:doi/10.1016%2FS0731-7085%2803%2900172-9&rft.externalDBID=n%2Fa&rft.externalDocID=15055145
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0731-7085&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0731-7085&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0731-7085&client=summon