Chemometric determination of amiloride hydrochloride, atenolol, hydrochlorothiazide and timolol maleate in synthetic mixtures and pharmaceutical formulations

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 34; no. 2; pp. 305 - 314
• Main Authors: Ferraro, Mónica C.F, Castellano, Patricia M, Kaufman, Teodoro S
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.02.2004; Elsevier Science
• Subjects: Amiloride - analysis; Amiloride - chemistry; Amiloride hydrochloride; Analysis; Analytical, structural and metabolic biochemistry; Atenolol; Atenolol - analysis; Atenolol - chemistry; Biological and medical sciences; Chemistry, Pharmaceutical; Chemometric determination; Fundamental and applied biological sciences. Psychology; General pharmacology; Hydrochlorothiazide; Hydrochlorothiazide - analysis; Hydrochlorothiazide - chemistry; Least-Squares Analysis; Medical sciences; Multivariate methods; Pharmacology. Drug treatments; Spectrophotometry, Ultraviolet - methods; Timolol - analysis; Timolol - chemistry; Timolol maleate; Timolol maleate; Hydrochlorothiazide; Amiloride hydrochloride; Atenolol; Chemometric determination; Multivariate methods; Antiglaucomatous agent; u-Plasminogen activator; Potassium sparing diuretic; Serine endopeptidases; Enzyme; Enzyme inhibitor; β-Adrenergic receptor; Peptidases; β1-Adrenergic receptor; Amiloride; Dosage form; Hydrolases; Antihypertensive agent; Antagonist; Thiazide; Timolol; Beta blocking agent; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(03)00521-1

Different chemometric methods such as classical least squares (CLS), principal components regression (PCR) and partial least squares with one dependent variable (PLS-1) applied on UV spectral data ( 0 D) and on their first derivatives ( 1 D) were evaluated for the simultaneous quantification of samples containing mixtures of amiloride hydrochloride, atenolol, hydrochlorothiazide and timolol maleate. Their performances were compared by means of ANOVA tests, which evidenced that 0 D-PCR, 0 D-PLS-1, 1 D-PCR and 1 D-PLS-1 were reproducible and gave statistically similar results, while 0 D-CLS and 1 D-CLS displayed higher variances than the former and failed to comply with the Levene’s variance homogeneity test at different stages of the method comparison and validation process. The four statistically equivalent procedures were successfully applied to the analysis of synthetic samples with two to four analytes and to commercial tablet preparations containing amiloride hydrochloride and hydrochlorothiazide alone or in association with atenolol or timolol maleate.