Tumour suppressor gene p53 in the horse: identification, cloning, sequencing and a possible role in the pathogenesis of equine sarcoid

The tumour suppressor protein p53 enhances the genetic stability of the cell and plays a critical role in tumour suppression. Equine p53 was analysed by sequencing exons 5 to 9, a region which includes most known mutations and all the mutational hotspots in the species that have been investigated. T...

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Published inResearch in veterinary science Vol. 61; no. 2; pp. 114 - 119
Main Authors Bucher, K, Szalai, G, Marti, E, Griot-Wenk, M.E, Lazary, S, Pauli, U
Format Journal Article
LanguageEnglish
Published England Elsevier India Pvt Ltd 01.09.1996
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cats
Cattle
Cercopithecus aethiops
Chickens
Cloning, Molecular
Cricetinae
DNA Primers
Exons
Genes, p53
Horse Diseases
Horses - genetics
Humans
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Rats
Sarcoma - genetics
Sarcoma - veterinary
Sequence Homology, Amino Acid
Sheep
Skin Neoplasms - genetics
Skin Neoplasms - veterinary
Trout
Tumor Suppressor Protein p53 - biosynthesis
Tumor Suppressor Protein p53 - chemistry
Tumor Suppressor Protein p53 - genetics
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Summary:The tumour suppressor protein p53 enhances the genetic stability of the cell and plays a critical role in tumour suppression. Equine p53 was analysed by sequencing exons 5 to 9, a region which includes most known mutations and all the mutational hotspots in the species that have been investigated. The fragment was amplified, cloned and sequenced from genomic and complementary DNA. A comparison of the predicted amino acid sequences between the horse and other species resulted in identities between 66 per cent with the clawed frog and 92 per cent with the cat. Using the single strand conformation polymorphism technique, exons 5 to 8 amplified from sarcoid tissue and peripheral leucocytes of 28 sarcoid-affected and 11 healthy horses were screened for mutations. No mutations were identified, suggesting that the frequency of p53 mutations in equine sarcoid might be low. However, the high incidence of bovine papillomavirus ( BPV) infection in equine sarcoid may indicate the functional inactivation of p53 by BPV-encoded E6 protein.
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ISSN:0034-5288
1532-2661
DOI:10.1016/S0034-5288(96)90084-3