FACS analysis—a new and accurate tool in the diagnosis of lymphoma in the cerebrospinal fluid

• Published in: Clinica chimica acta Vol. 317; no. 1; pp. 101 - 107
• Main Authors: Urbanits, Sabine, Griesmacher, Andrea, Hopfinger, Georg, Stockhammer, Günther, Karimi, Alireza, Müller, Mathias M., Pittermann, Elisabeth, Grisold, Wolfgang
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.03.2002; Elsevier
• Subjects: Adult; Aged; Biological and medical sciences; CSF analysis; CSF involvement; FACS; Female; Flow Cytometry - methods; Fluorescence activated cell scanning; Hematologic and hematopoietic diseases; Humans; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma; Lymphoma - cerebrospinal fluid; Lymphoma - diagnosis; Lymphoma - immunology; Medical sciences; Middle Aged; CSF analysis; FACS; Lymphoma; Fluorescence activated cell scanning; CSF involvement; Human; Cytometry; Fluorescent labelling; Lymphoproliferative syndrome; Exploration; Malignant hemopathy; Diagnosis; Cerebrospinal fluid; Tumor cell
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/S0009-8981(01)00771-9

Background: Fluorescence activated cell scanning (FACS) is a useful tool for identifying malignant cell clones of lymphoma cells in cerebrospinal fluid (CSF) by immunological phenotype. Methods: We used FACS analysis for demonstrating it to be a quick and reliable technology that is available in most hematological laboratories. In this study, we demonstrate the clinical application of FACS analysis within a series of 15 lymphoma patients with suspected CSF involvement. CSF from three patients with another diagnosis than lymphoma serves as negative control. Results and conclusion: A malignant cell clone cannot only be identified in CSF phenotypically, but also classified according to the immunological surface profile. As this method improves the diagnostic sensitivity and specificity, it should be implemented into routine diagnosis.