Multiple target sites of allelic imbalance on chromosome 17 in Barrett's oesophageal cancer

• Published in: Oncogene Vol. 18; no. 4; pp. 987 - 993
• Main Authors: DUNN, J, GARDE, J, DOLAN, K, GOSNEY, J. R, SUTTON, R, MELTZER, S. J, FIELD, J. K
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 28.01.1999
• Subjects: Adenocarcinoma - genetics; Barrett Esophagus - genetics; Biological and medical sciences; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Chromosome Mapping; Chromosomes, Human, Pair 17 - genetics; Esophageal Neoplasms - genetics; Fundamental and applied biological sciences. Psychology; Genes, p53 - genetics; Genes, Tumor Suppressor - genetics; Genetic Markers; Humans; Loss of Heterozygosity; Microsatellite Repeats - genetics; Molecular and cellular biology; Adenocarcinoma; Genetic mapping; Human; Chromosome E17; Esophageal disease; Loss of heterozygosity; Deletion; Digestive diseases; Tumor; Barrett esophagus; Tumor suppressor gene
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1202371

Twelve Barrett's adenocarcinomas have been analysed for the occurrence of allelic imbalance (LOH) on chromosome 17 using 41 microsatellite markers. This study provides evidence for 13 minimal regions of LOH, six on 17p and seven on 17q. Four of these centre in the vicinity of the known tumour suppressor genes (TSGs) TP53 (17p13.1), NFI (17q11.2), BRCA1 (17q21.1), and a putative TSG (17p13.3). The tumours all displayed relatively small regions of LOH (1-10 cM), and in several tumours extensive regions of LOH were detected. One tumour displayed only two very small regions of LOH; 17p11.2 and 17p13.1. The frequency of allelic imbalance has been calculated based on the LOH encompassing only one minimal region, and based on all the LOH observations. By both evaluations the highest LOH frequencies were found for regions II (p53), III (17p13.1 centromeric to p53), IV (17p12), V (17p11.2) and VII (NF1, 17q11.2). Our data supports the existence of multiple TSGs on chromosome 17 and challenges the view that p53 is the sole target of LOH on 17p in Barrett's adenocarcinoma.