Sue1p Is Required for Degradation of Labile Forms of Altered Cytochromes c in Yeast Mitochondria
Previous studies on certain altered holo-isocytochromes c revealed a Ï â -dependent degradation (RDD) phenotype, in which certain altered holo-iso-1-cytochromes c are at normal or nearly normal levels in Ï + strains, but are at low levels or absent in Ï â strains, although wild-type holo-iso-...
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Published in | The Journal of biological chemistry Vol. 279; no. 29; pp. 30449 - 30458 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
16.07.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Previous studies on certain altered holo-isocytochromes c revealed a Ï â -dependent degradation (RDD) phenotype, in which certain altered holo-iso-1-cytochromes c are at normal or nearly normal levels in Ï + strains, but are at low levels or absent in Ï â strains, although wild-type holo-iso-1-cytochrome c is present at normal levels in both Ï + and related Ï â strains. The diminished levels of altered holo-iso-1-cytochrome c are due to the rapid degradation that is carried out by a novel proteolytic pathway in the IMS of mitochondria. SUE1 , a nuclear gene that encodes a mitochondrial protein, was identified with a genetic screen for mutants that diminish RDD.
The levels of RDD and certain other types of altered holo-iso-1-cytochrome c were elevated in Ï â sue1 strains. Also, Ï + sue1 strains containing certain altered holo-iso-1-cytochromes c grew better on non-fermentable carbon sources than the corresponding Ï + SUE1 strains. These results indicate that Sue1p may play an important role in the degradation of abnormal holo-iso-1-cytochrome
c in the mitochondria. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M403742200 |