Regulators of G-protein Signaling (RGS) 4, Insertion into Model Membranes and Inhibition of Activity by Phosphatidic Acid
Regulators of G-protein signaling (RGS) proteins are critical for attenuating G protein-coupled signaling pathways. The membrane association of RGS4 has been reported to be crucial for its regulatory activity in reconstituted vesicles and physiological roles in vivo . In this study, we report that R...
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Published in | The Journal of biological chemistry Vol. 278; no. 13; pp. 11115 - 11122 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
28.03.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Regulators of G-protein signaling (RGS) proteins are critical for attenuating G protein-coupled signaling pathways. The membrane
association of RGS4 has been reported to be crucial for its regulatory activity in reconstituted vesicles and physiological
roles in vivo . In this study, we report that RGS4 initially binds onto the surface of anionic phospholipid vesicles and subsequently inserts
into, but not through, the membrane bilayer. Phosphatidic acid, one of anionic phospholipids, could dramatically inhibit the
ability of RGS4 to accelerate GTPase activity in vitro . Phosphatidic acid is an effective and potent inhibitor of RGS4 in a Gα i1 -[γ- 32 P]GTP single turnover assay with an IC 50 ⼠4 μ m and maximum inhibition of over 90%. Furthermore, phosphatidic acid was the only phospholipid tested that inhibited RGS4 activity
in a receptor-mediated, steady-state GTP hydrolysis assay. When phosphatidic acid (10 mol %) was incorporated into m1 acetylcholine
receptor-Gα q vesicles, RGS4 GAP activity was markedly inhibited by more than 70% and the EC 50 of RGS4 was increased from 1.5 to 7 n m . Phosphatidic acid also induced a conformational change in the RGS domain of RGS4 measured by acrylamide-quenching experiments.
Truncation of the N terminus of RGS4 (residues 1â57) resulted in the loss of both phosphatidic acid binding and lipid-mediated
functional inhibition. A single point mutation in RGS4 (Lys 20 to Glu) permitted its binding to phosphatidic acid-containing vesicles but prevented lipid-induced conformational changes
in the RGS domain and abolished the inhibition of its GAP activity. We speculate that the activation of phospholipase D or
diacylglycerol kinase via G protein-mediated signaling cascades will increase the local concentration of phosphatidic acid,
which in turn block RGS4 GAP activity in vivo . Thus, RGS4 may represent a novel effector of phosphatidic acid, and this phospholipid may function as a feedback regulator
in G protein-mediated signaling pathways. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M212606200 |