Selenium, glutathione peroxidases, and some other antioxidant parameters in blood of patients with chronic renal failure

• Published in: Journal of trace elements in medicine and biology Vol. 15; no. 2; pp. 161 - 166
• Main Authors: Zachara, Bronisław A., Trafikowska, Urszula, Adamowicz, Andrzej, Nartowicz, Edmund, Manitius, Jacek
• Format: Journal Article
• Language: English
• Published: Stuttgart Elsevier GmbH 2001; Fischer
• Subjects: Adult; Aged; Antioxidants - metabolism; Biological and medical sciences; blood; Erythrocytes - metabolism; Female; Glutathione Peroxidase - blood; Glutathione Peroxidase - metabolism; Humans; kidney; Kidney - metabolism; Kidney Failure, Chronic - blood; Male; Malondialdehyde - blood; malonyldialdehyde; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Oxidative Stress; plasma; red cells; Renal failure; Selenium - blood; superoxide dismutase; trace elemets; uremic patients; kidney; malonyldialdehyde; uremic patients; superoxide dismutase; trace elemets; plasma; blood; red cells; Kidney disease; Glutathione peroxidase; Human; Urinary system disease; Biochemical analysis; Enzyme; Red blood cell; Superoxide dismutase; Antioxidant; Blood; Blood plasma; Trace element; Chronic; Peroxidases; Renal failure; Oxidoreductases; Selenium; Comparative study; Quantitative analysis
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/S0946-672X(01)80061-4

In the present study several parameters associated with oxidative stress were examined in the blood of 25 chronic renal failure (CRF) patients and the results were compared with 18 healthy subjects. Mean creatinine concentration in patients was 1216 ± 292 μmol/l. Selenium (Se) concentration in red cells, whole blood and in plasma of CRF patients (106 ± 32.5, 59.0 ± 16.7 and 42.4 ± 13.8 ng/ml, respectively) was significantly (0.0001 < P < 0.01) lower (by 20–42%) compared with the controls. Red cell and plasma glutathione peroxidase (GSH-Px) activities (16.6 ± 3.4 U/g Hb and 93.7 ± 32.9 U/l plasma) were lower by 12 and 53% (P < 0.05 and < 0.0001, respectively) in patients than in healthy subjects. GSH concentration in red cells of patients (2.81 ± 0.45 mmol/l) was significantly (P < 0.001) higher (by 20%) than in control group. Malonyldialdehyde (MDA) concentration (expressed as thiobarbituric acid-reactive substances) in red cells of patients (725 ± 155 nmol/g Hb) was significantly (P < 0.001) higher (by 28%) than in control group. No significant difference was observed in the activity of superoxide dismutase in plasma between the two groups. In conclusion, our results confirm that the alterations in Se levels in blood components and in GSH-Px activity in plasma show that the kidney plays an important role in Se homeostasis and in plasma GSH-Px synthesis.