Antimicrobial and Cytotoxic Activity of Endophytic Fungi from Lagopsis supina

In this study, five endophytic fungi belonging to the and genera were isolated from . The antimicrobial activity of all fungal fermented extracts against and was tested using the cup-plate method. Among them, XZC-1 showed the best activity and was subsequently selected for large-scale fermentation a...

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Published inJournal of microbiology and biotechnology Vol. 33; no. 4; pp. 543 - 551
Main Authors Zhang, Dekui, Sun, Weijian, Xu, Wenjie, Ji, Changbo, Zhou, Yang, Sun, Jingyi, Tian, Yutong, Li, Yanling, Zhao, Fengchun, Tian, Yuan
Format Journal Article
LanguageEnglish
Published Korea (South) The Korean Society for Microbiology and Biotechnology 28.04.2023
Subjects
Anti-Bacterial Agents - metabolism
Anti-Bacterial Agents - pharmacology
Anti-Infective Agents - metabolism
Antifungal Agents - metabolism
Antineoplastic Agents - metabolism
Antineoplastic Agents - pharmacology
Aspergillus
Endophytes
Fungi
Microbial Sensitivity Tests
Research article
Staphylococcus aureus
Endophytic fungi
antimicrobial activity
cytotoxic activity
Lagopsis supina
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Summary:In this study, five endophytic fungi belonging to the and genera were isolated from . The antimicrobial activity of all fungal fermented extracts against and was tested using the cup-plate method. Among them, XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2-methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against , , , , , , and with diverse MIC values (128-512 μg/ml), which were close to that of the positive control antifungal, actidione (64-128 μg/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against , , , and , with low MIC values (8-64 μg/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.
Bibliography:Dekui Zhang, Weijian Sun and Wenjie Xu contributed equally to this study.
ISSN:1017-7825
1738-8872
DOI:10.4014/jmb.2211.11055