Simultaneous enantioseparation of cis-diltiazem hydrochloride and its metabolite cis-desacetyldiltiazem using high-performance liquid chromatography and capillary electrophoresis

Two alternative methods were developed using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) for a simultaneous enantioselective determination of Ca 2+ blocking chiral drug cis-diltiazem hydrochloride (DLT) and its degradation product and metabolite cis-desacetyldilt...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 27; no. 1; pp. 161 - 166
Main Authors Chankvetadze, Bezhan, Kartozia, Irma, Blaschke, Gottfried
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 2002
Elsevier Science
Subjects
Analysis
Biological and medical sciences
Calcium Channel Blockers - isolation & purification
Capillary electrophoresis
Chiral selector
Chromatography, High Pressure Liquid - methods
cis-Desacetyldiltiazem
cis-Diltiazem hydrochloride
Cyclodextrin
Diltiazem - analogs & derivatives
Diltiazem - isolation & purification
Diltiazem - metabolism
Electrophoresis, Capillary - methods
Enantioseparation
General pharmacology
HPLC
Mass Spectrometry
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Stereoisomerism
Chiral selector
Cyclodextrin
Enantioseparation
Capillary electrophoresis
cis-Diltiazem hydrochloride
cis-Desacetyldiltiazem
HPLC
Separation
Calcium antagonist
Benzothiazepine derivatives
HPLC chromatography
Diltiazem
Electrospray
Analysis method
Enantiomer
Simultaneous measurement
Mass spectrometry
Comparative study
Quantitative analysis
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Summary:Two alternative methods were developed using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) for a simultaneous enantioselective determination of Ca 2+ blocking chiral drug cis-diltiazem hydrochloride (DLT) and its degradation product and metabolite cis-desacetyldiltiazem (Desac-DLT). The methods were compared from the viewpoint of analytical performance. The identification of HPLC peaks were performed using off-line electrospray ionization mass spectrometry. In addition, CE method was evaluated for quantitative determination of minor enantiomeric impurities in pharmacologically active (+)- cis-DLT.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(01)00553-2