Enhancement of tumorigenic, metastatic and in vitro invasive capacity of rat mammary tumor cells by transforming growth factor-β

• Published in: Cancer letters Vol. 175; no. 1; pp. 95 - 106
• Main Authors: Nakata, Daichi, Hamada, Jun-ichi, Ba, Yi, Matsushita, Kazuhiro, Shibata, Toshiyuki, Hosokawa, Masuo, Moriuchi, Tetsuya
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 10.01.2002; Elsevier
• Subjects: Adhesion; Animals; Antibodies - pharmacology; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; CD44; Cell Adhesion - drug effects; Cell Adhesion Molecules - genetics; Cell Movement - drug effects; Chemical agents; Chemotaxis - drug effects; Dose-Response Relationship, Drug; Female; Hyaluronan Receptors - immunology; Hyaluronic Acid - pharmacology; Hyaluronoglucosaminidase - pharmacology; Immediate-Early Proteins - genetics; Invasion; Kinetics; Mammary Neoplasms, Experimental - pathology; Medical sciences; Metastasis; Mice; Motility; Neoplasm Invasiveness; Neoplasm Metastasis - pathology; Nuclear Proteins - genetics; Trans-Activators - genetics; Transforming Growth Factor beta - pharmacology; Transforming growth factor-β; Tumors; Xenopus Proteins; Motility; Metastasis; Adhesion; Transforming growth factor-β; Invasion; CD44; Adenocarcinoma; Rat; Transforming growth factor β; Cell cell interaction; Malignancy; Potential; Established cell line; Tumor progression; Mammary gland; Mechanism of action; Tumor cell; Rodentia; Tumorigenicity; Malignant tumor; In vitro; Mammary gland diseases; Vertebrata; Mammalia; Animal; Low malignancy; Metastatic
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(01)00728-5

We investigated the effects of transforming growth factor-β (TGF-β) on biological behavior of a weakly malignant rat mammary carcinoma ER-1 cell line. TGF-β enhanced the tumorigenic and metastatic capacity of ER-1 cells and their in vitro invasiveness to rat mesothelial and endothelial cell. Further cell biological analysis indicated that the increased invasive and metastatic capacity of ER-1 cells by TGF-β was due to the increase in cell motility and adhesion to the mesothelial and endothelial cell monolayers. Thus, it is suggested that TGF-β acts on ER-1 cells as a progression-enhancing factor which stimulates their adhesive and motile activities.