An ultrasensitive tumor enriched flow-cytometric assay for detection of isolated tumor cells in bone marrow of patients with breast cancer
An ultrasensitive tumor enriched flow-cytometric assay was used to determine its feasibility in detection of isolated tumor cells (ITC) in bone marrow (BM) of patients with breast cancer. Epithelial cells were removed by magnetic microbeads conjugated with an anti-cytokeratin 7/8 monoclonal antibody...
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Published in | The American journal of surgery Vol. 184; no. 5; pp. 414 - 417 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.11.2002
Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | An ultrasensitive tumor enriched flow-cytometric assay was used to determine its feasibility in detection of isolated tumor cells (ITC) in bone marrow (BM) of patients with breast cancer.
Epithelial cells were removed by magnetic microbeads conjugated with an anti-cytokeratin 7/8 monoclonal antibody to enrich tumor cells in BM samples. A specific gate for MCF-7 breast cancer cells (gate
MCF-7 cells) was also taken into consideration in addition to a gate including all enriched BM cells (gate
enriched BM cells) in flow-cytometric analysis to enhance the specificity of the method.
Nineteen patients with stage I/II were evaluated. Ten patients (53%) were found to have cytokeratin positive (CK
+) cells according to the gate
enriched BM cells whereas 6 patients (32%) had CK
+ cells when the gate
MCF-7 cells was taken into account.
New strategies in nonmorphological ultrasensitive techniques might be useful to categorize patients with ITCs having different tumor morphology and characteristics. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0002-9610 1879-1883 |
DOI: | 10.1016/S0002-9610(02)01015-2 |