An Amperometric Xanthine Oxidase Enzyme Electrode Based on Hydrogen Peroxide Electroreduction

A xanthine oxidase enzyme electrode (xanthine oxidase immobilized on electrochemically modified graphite and conveniently coated with gelatine electrode working surface) for quantitative analysis of xanthine is proposed. The detection of thus developed electrochemical system is based on the electror...

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Published inZeitschrift für Naturforschung C. A journal of biosciences Vol. 57; no. 9; pp. 883 - 889
Main Authors Dimcheva, Nina, Horozova, Elena, Jordanova, Zinaida
Format Journal Article
LanguageEnglish
Published Germany Verlag der Zeitschrift für Naturforschung 01.09.2002
Subjects
Animals
Electrochemistry
Enzyme Electrode
Enzymes, Immobilized - metabolism
Graphite
Hydrogen Peroxide
Hydrogen Peroxide Electroreduction
Kinetics
Milk - enzymology
Oxidation-Reduction
Xanthine Oxidase
Xanthine Oxidase - metabolism
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Summary:A xanthine oxidase enzyme electrode (xanthine oxidase immobilized on electrochemically modified graphite and conveniently coated with gelatine electrode working surface) for quantitative analysis of xanthine is proposed. The detection of thus developed electrochemical system is based on the electroreduction of hydrogen peroxide generated in enzyme layer and offered l-ascorbic and uric acid reducing interference effect on the substrate determination. At a working potential −50 mV (vs. Ag/AgCl) the detection limit of 4.5 μm and the linearity of the amperometric signal up to substrate concentration of about 40 μm were found. At that working potential, the electrode is practically inert towards l-ascorbic- and uric acid present. The response time did not exceed 2 min.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0939-5075
1865-7125
DOI:10.1515/znc-2002-9-1021