Monitoring fermentation media by ion-chromatography with a double chamber bulk acoustic wave detector

• Published in: Talanta (Oxford) Vol. 50; no. 5; pp. 1019 - 1025
• Main Authors: Xie, You-tao, Zeng, Hong-shan, Yao, Shou-zhuo, Wei, Wan-zhi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.12.1999; Oxford Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Double chamber bulk acoustic wave detector; Fermentation; Fundamental and applied biological sciences. Psychology; Ion-chromatography; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Monitor; Ion-chromatography; Double chamber bulk acoustic wave detector; Monitor; Fermentation; Acoustic wave device; Culture medium; Sodium Ions; Escherichia coli; Bulk wave; Ammonium Ions; Potassium Ions; Ion exchange chromatography; Microorganism culture; Magnesium Ions; Bacteria; Evolution; Detection; Monitoring; Enterobacteriaceae; Quantitative analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/S0039-9140(99)00211-8

A reliable ion-chromatographic (IC) method with a novel double chamber bulk acoustic wave (DCBAW) detector was developed for monitoring five important inorganic cations (Na +, K +, NH 4 +, Ca 2+, Mg 2+) in biological culture media. A Shimpack IC-C1 analytical column with 5 mM hydrochloric acid and 2 mM acetonitrile as mobile phase was used. All investigated inorganic cations could be detected and qualified in the range of 0.1–100 mg/l. Results showed that the consumption velocities of Mg 2+ and K + are related to the growth of the cells and decrease fastest during the first 2–5 h. Mg became a growth limiting factor at concentration below 0.1 mg/l. The concentrations of the other cations stayed nearly constant during the whole fermentation process. The simple sample preparation, short analytical time and accurate results made it a useful tool for the on-line monitoring, controlling and optimization of the fermentation process.