Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system

•An allelic knockout model of dHAND in hESCs-H9 was established (dHAND-KO).•dHAND-KO hESC line could differentiate into all three germ layers in vivo.•dHAND-KO hESC line provides an opportunity to study the functions of dHAND. In order to determine the function of dHAND in a specific subset of cardi...

Full description

Saved in:
Bibliographic Details
Published inStem cell research Vol. 55; p. 102471
Main Authors Wang, Wei, Bai, Rui, Song, Xiantao
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.08.2021
Elsevier
Online AccessGet full text

Cover

More Information
Summary:•An allelic knockout model of dHAND in hESCs-H9 was established (dHAND-KO).•dHAND-KO hESC line could differentiate into all three germ layers in vivo.•dHAND-KO hESC line provides an opportunity to study the functions of dHAND. In order to determine the function of dHAND in a specific subset of cardiomyocyte progenitor cells responsible for the heart and its function in the cellular mechanism of Hand2−/− mouse ventricular hypoplasia, we established an allelic knockout model of dHAND in human embryonic stem cells (hESCs-H9) by an episomal vector-based CRISPR/Cas9 system. This dHAND KO hESC line maintained normal karyotype and typical primed pluripotent human stem cell morphology, and maintained pluripotency, could differentiate into all three germ layers in vivo.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2021.102471