Nanohybrid Based on (Mn, Zn) Ferrite Nanoparticles Functionalized With Chitosan and Sodium Alginate for Loading of Curcumin Against Human Breast Cancer Cells

• Published in: AAPS PharmSciTech Vol. 24; no. 8; p. 222
• Main Authors: Ahmadi, Fatemeh, Saeedi, Majid, Akbari, Jafar, Seyedabadi, Mohammad, Ebrahimnejad, Pedram, Morteza-Semnani, Katayoun, Ghasemi, Shahram, Moalem-Banhangi, Monire, Babaei, Amirhossein, Hashemi, Seyyed Mohammad Hassan, Asare-Addo, Kofi, Nokhodchi, Ali
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 07.11.2023
• Subjects: Alginates - chemistry; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Breast Neoplasms - drug therapy; Chitosan - chemistry; Curcumin - chemistry; Curcumin - pharmacology; Drug Carriers - chemistry; Female; Humans; Nanoparticles - chemistry; Particle Size; Pharmacology/Toxicology; Pharmacy; Research Article; Zinc; alginate; chitosan; breast cancer; drug release; magnetic nanoparticles; curcumin
• ISSN: 1530-9932; 1530-9932
• DOI: 10.1208/s12249-023-02683-9

This study reports on the synthesis of Mn 1 − x Zn x Fe 2 O 4 (Mn, Zn ferrite) magnetic nanoparticles (MNPs) as drug delivery carriers for effective therapeutic outcomes. The MNPs were prepared using the coprecipitation method, and their magnetic properties were investigated based on their composition. Among the compositions tested, Mn 0.8 Zn 0.2 Fe 2 O 4 MNPs exhibited superparamagnetic properties with a saturation magnetization moment of 34.6 emu/g at room temperature (25°C). To enhance the water solubility of curcumin (Cur), known for its hydrophobic nature, it was successfully loaded onto alginate (Alg)/chitosan (Chit)@Mn 0.8 Zn 0.2 Fe 2 O 4 nanoparticles (NPs). The nanocomposite was characterized by field emission scanning electron microscopy (FE-SEM) which revealed a particle size of approximately 20 nm. The crystalline structure of the NPs was analyzed using X-ray diffraction, while Fourier-transform infrared (FTIR), energy-dispersive X-ray, and map analysis techniques were employed for further characterization. In terms of drug release, there was an initial burst release of Cur (around 18%) within the first hour, followed by a slower release (approximately 61%) over the next 36 h. The anti-tumor properties of the Cur-loaded NPs were evaluated using the Methyl Thiazol Tetrazolium (MTT) assay and quantitative real-time polymerase chain reaction. The MTT assay confirmed a higher cytotoxic effect of Cur-loaded Alg/Chit@Mn 0.8 Zn 0.2 Fe 2 O 4 NPs on the MCF-7 breast cancer cell line compared to free Cur, highlighting the significance of incorporating Cur into nano-sized carrier systems. Graphical Abstract