Handling and detection of 0.8 amol of a near-infrared cyanine dye by capillary electrophoresis with laser-induced fluorescence detection

• Published in: Journal of Chromatography A Vol. 979; no. 1; pp. 307 - 314
• Main Authors: Yang, Changming, Shimelis, Olga, Zhou, Xiaojuan, Li, Guoding, Bayle, Christophe, Nertz, Michel, Lee, Hyeran, Strekowski, Lucjan, Patonay, Gabor, Couderc, Francois, Giese, Roger W
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 06.12.2002; Elsevier
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbocyanines - analysis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Coloring Agents - analysis; Cyanine dyes; DNA; Dna, deoxyribonucleoproteins; Dyes; Electrophoresis, Capillary - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Lasers; Magnetic Resonance Spectroscopy; Nucleic acids; Other chromatographic methods; Spectrometry, Fluorescence - methods; Spectroscopy, Near-Infrared; Cyanine dyes; Detection, electrophoresis; Dyes; DNA; Trace analysis; Fluorescence detector; Zone electrophoresis; Capillary electrophoresis; Laser induced fluorescence; Experimental study; Cyanine dye; Near infrared radiation; Infrared laser; Fluorescent labelling; Detection
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)01441-3

We are interested in the detection of DNA adducts and other trace analytes by labeling them with a fluorescent tag followed by use of capillary electrophoresis with laser-induced fluorescence detection (CE–LIF) for high resolution and sensitivity. Towards this goal, here we report the following: (1) synthesis and handling properties of a near-IR, carboxyl-substituted heptamethine cyanine dye; (2) modification of an existing ball lens LIF detector to provide near-LIF detection with excitation at 785 nm for CE; and (3) corresponding handling and detection of as little as 0.8 amol of the dye by enrich-injection of 4.7 μl of 1·10 −13 mol/l dye in methanol from an 8-μl volume into a corresponding CE–LIF system. The electrolyte for the separation was methanol–40 mmol/l aqueous sodium borate (98:2, v/v). This finding encourages further exploration of the dye by functionalization of its carboxyl group for chemical labeling purposes.