Cultivation of the marine basidiomycete Nia vibrissa (Moore & Meyers)

• Published in: Journal of biotechnology Vol. 70; no. 1; pp. 203 - 206
• Main Authors: Helmholz, H., Etoundi, P., Lindequist, U.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 30.04.1999; Amsterdam Elsevier; New York, NY
• Subjects: Basidiomycetes; Biological and medical sciences; Biotechnology; cell culture; culture media; Endotoxin; Fundamental and applied biological sciences. Psychology; Marine fungi; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Nia vibrissa; Marine fungi; Endotoxin; Nia vibrissa; Culture medium; Microorganism growth; Basidiomycetes; Environmental factor; Biological activity; Glycolipid; Fungi; Marine environment; Production; Microorganism culture; Isolate; Antagonist; Thallophyta
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/S0168-1656(99)00073-5

An isolate of Nia vibrissa was fermented in liquid shake cultures and on agar. Suitable cultivation conditions are a prerequisite for the continuous synthesis of biological active metabolites. The growth response of N. vibrissa to four selected media and several environmental factors, such as salinity, pH and light, was studied. The amount of produced mycelia and the quantity and activity of the organic extracts were parameters for the optimal cultivation method. The ethanolic extracts of the mycelia of N. vibrissa grown in all the investigated nutrient media, showed an influence of the duration of cultivation on the biological activity. A synthetic medium with a pH of 7.5 was the preferred nutrient medium. The addition of wood and incubation under continuous light had no effect on growth but increased the activity of the ethanolic extract. The optimal agar medium salinity for colony growth was in the range between 5 and 25‰. At a salinity of 150‰ growth was not observed.