CD66 Identifies a Neutrophil-Specific Epitope Within the Hematopoietic System That Is Expressed by Members of the Carcinoembryonic Antigen Family of Adhesion Molecules

• Published in: Blood Vol. 78; no. 1; pp. 63 - 74
• Main Authors: Watt, Suzanne M., Sala-Newby, Graciela, Hoang, Trang, Gilmore, David J., Grunert, Fritz, Nagel, Gerhard, Murdoch, Sarah J., Tchilian, Elma, Lennox, E.S., Waldmann, Herman
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.1991
• Subjects: Antibodies, Monoclonal - immunology; Antigens, CD - analysis; Antigens, CD - immunology; Antigens, Differentiation - analysis; Antigens, Differentiation - immunology; Binding, Competitive; Blotting, Western; Bone Marrow - immunology; Bone Marrow Cells; Carcinoembryonic Antigen - immunology; Cell Adhesion Molecules - immunology; Epitopes - immunology; Flow Cytometry; HeLa Cells; Hematopoietic System - immunology; Humans; Leukocytes - cytology; Leukocytes - immunology; Neutrophils - immunology; Transfection
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V78.1.63.63

Preliminary results from the IVth Leucocyte Culture Conference have classified the monoclonal antibody (MoAb), YTH 71.3.2, as CD66. Two other MoAbs, YPC 2/12.1 and CE6/ 2D3.1, share a common cellular specificity, reacting with cells of the neutrophil series and colonic epithelium. The YTH 71.3.2 and CE6/2D3.1 MoAbs both recognize a similar CD66 defined epitope that is distinct from that identified by YPC 2/12.1. By Western blotting, these antibodies react with different molecular species from cells of different lineages. The antibodies identify 50- to 55-Kd, 80- to 100-Kd, and 130-to 200-Kd components present in a semi-purified carcinoembryonic antigen (CEA) preparation from colonic adenocarcinomas and a 90- to 130-Kd molecule from HL-60 cells. With the colonic cell line, LS174T, YPC2/12.1 stains diffuse bands of 160 to 200 Kd and 90 to 130 Kd with equal intensity, whereas the binding of CE6/2D3.1 and YTH 71.3.2 is biased toward the lower molecular weight set of molecules. Remarkably, all three antibodies recognize CEA-related molecules. Defined analyses using HeLa cells transfected with CEA, NCA(NCA-50/ 90), and CGM6(NCA-95) cDNAs show that the three MoAbs identify CEA to varying degrees. While YTH 71.3.2 and CE6/2D3.1 also bind to NCA-50/90, YPC 2/12.1 recognizes an epitope expressed by both the NCA-50/90 and NCA-95 molecular species.