A Simple Parallel Analytical Method of Prenatal Screening

• Published in: Gynecologic and obstetric investigation Vol. 62; no. 4; pp. 220 - 225
• Main Authors: Li, Ding, Yang, Hao, Zhang, Wen-Hong, Pan, Hao, Wen, Dong-Qing, Han, Feng-Chan, Guo, Hui-Fang, Wang, Xiao-Ming, Yan, Xiao-Jun
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2006; S. Karger AG
• Subjects: Biological and medical sciences; Cytomegalovirus; Dose-Response Relationship, Drug; Female; Gynecology. Andrology. Obstetrics; Herpes simplex virus 1; Human protozoal diseases; Humans; Immunoglobulin G - blood; Infectious diseases; Linear Models; Medical sciences; Original Article; Parasitic diseases; Pregnancy; Prenatal Diagnosis - methods; Protein Array Analysis - economics; Protein Array Analysis - methods; Protozoal diseases; Reproducibility of Results; Rubella virus; Toxoplasma gondii; Toxoplasmosis; Cytomegalovirus; Rubella; ToRCH diagnosis; Toxoplasmosis; Herpes simplex virus type 1 and 2; Dot immunogold filtration assay; Microarray; Protein; Protozoal disease; Immunogold labelling; Alphaherpesvirinae; DNA chip; Betaherpesvirinae; Assay; Human herpesvirus 1; Filtration; Herpesviridae; Prenatal Diagnosis; Gynecology; Parasitosis; Method; Medical screening; Obstetrics; Infection; Virus; Viral disease
• ISSN: 0378-7346; 1423-002X
• DOI: 10.1159/000094092

Protein microarray has progressed rapidly in the past few years, but it is still hard to popularize it in many developing countries or small hospitals owing to the technical expertise required in practice. We developed a cheap and easy-to-use protein microarray based on dot immunogold filtration assay for parallel analysis of ToRCH-related antibodies including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus type 1 and 2 in sera of pregnant women. It does not require any expensive instruments and the assay results can be clearly recognized by the naked eye. We analyzed 186 random sera of outpatients at the gynecological department with our microarray and commercial ELISA kit, and the results showed there was no significant difference between the two detection methods. Validated by clinical application, the microarray is easy to use and has a unique advantage in cost and time. It is more suitable for mass prenatal screening or epidemiological screening than the ELISA format.