Mitochondrial Perturbation Negatively Affects Auxin Signaling

• Published in: Molecular plant Vol. 7; no. 7; pp. 1138 - 1150
• Main Authors: Kerchev, Pavel Ivanov, De Clercq, Inge, Denecker, Jordi, Mühlenbock, Per, Kumpf, Robert, Nguyen, Long, Audenaert, Dominique, Dejonghe, Wim, Van Breusegem, Frank
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.07.2014
• Subjects: Acrylates - pharmacology; Arabidopsis - cytology; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis Proteins - genetics; auxin signaling; Indoleacetic Acids - metabolism; Mitochondria - drug effects; Mitochondria - metabolism; mitochondrial perturbation; Regulatory Sequences, Nucleic Acid - drug effects; Regulatory Sequences, Nucleic Acid - genetics; retrograde communication; Signal Transduction - drug effects; 信号传导; 合成化学; 扰动; 生物学方法; 生长素; 线粒体; 葡糖基转移酶; 转录物; auxin signaling; mitochondrial perturbation; retrograde communication
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/ssu071

Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial per- turbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a （2-furyl）acrylate （FAA） substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.