Ratiometric fluorescence detection of a tag fused protein using the dual-emission artificial molecular probe

We have successfully developed a ratiometric detection system for protein of interest using the complementary recognition pair of the tetra-aspartate peptide tag and the SNARF-appended Zn(ii)-DpaTyr probe.

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Bibliographic Details
Published inChemical communications (Cambridge, England) no. 38; p. 4024
Main Authors Honda, Kei, Nakata, Eiji, Ojida, Akio, Hamachi, Itaru
Format Journal Article
LanguageEnglish
Published England 01.01.2006
Subjects
Amino Acid Sequence
Benzopyrans - chemistry
Fluoresceins - chemistry
Fluorescent Dyes - chemistry
Hydrogen-Ion Concentration
Organometallic Compounds - chemistry
Proteins - analysis
Ribonucleases - analysis
Spectrometry, Fluorescence
Staining and Labeling - methods
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Summary:We have successfully developed a ratiometric detection system for protein of interest using the complementary recognition pair of the tetra-aspartate peptide tag and the SNARF-appended Zn(ii)-DpaTyr probe.
ISSN:1359-7345
DOI:10.1039/b608684e